Tuberculosis still has increased prevalence rate in India and Puducherry though there are pharmacological interventions available to treat Tuberculosis.² It is considered as serious social health problem as it is completely curable because it spreads easily if the preventive measures are not taken. Through this research study the factors were assessed by which measures to improve the quality of life and decrease the incidence and prevalence of TB can beachieved.³ A descriptive retrospective cohort research design was undertaken in various areas (Mudaliarpet, Muthialpet, Lawspet, Reddiarpalayam, etc.) of Puducherry. The data was collected from 100 subjects (defaulters) through purposive sampling technique to assess the factors affecting the non - compliance through structured questionnaires. Overall research findings showed that majority 93% were males, 34% were alcoholics and 27% were alcoholics with smoking. There was a significant association between the factors affecting non-compliance under DOTS regimen with selected demographic variables.