A partograph is one of the important tools to monitor the progress of labor and is also helpful for managing labor. Therefore, it should be used for all women during the first stage of labor admitted to a labor room. Partograph is needed for observations of the mother and fetus and also assists in early decision-making in the intranatal period, such as transfer, augmentation, and termination of labor. Aim of the study: The study was aimed to assess the knowledge and practice regarding the plotting of partograph. Materials and methods: The nonexperimental descriptive design was used for the study. Thirty staff nurses were selected by nonprobability purposive sampling technique. A structured knowledge questionnaire and practice checklist were used for data collection. Results of the study: The results revealed that the majority (53.3%; n = 16) of respondents were having average knowledge score, whereas 43.3% (13) of respondents had good knowledge scores, and only 3.3% (1) of respondents had a poor level of knowledge on partograph. Moreover, 70% (21) of respondents have inadequate practice, and 30% (9) of respondents have adequate practice. Conclusion: This study concluded that staff nurse has average knowledge regarding partograph but practicing plotting of partograph. Clinical significance: This study shows that staff nurses were having an adequate knowledge regarding partograph but do not adequately practicing the plotting of partograph. Therefore, it can use as a tool to monitor labor and prevent unnecessary problems for mothers and fetuses by reducing the total duration of labor.