Introduction: Natural extract dyes are eco-friendly and nonhazardous when compared with synthetic stains. Routinely, hematoxylin and eosin (H&E) stain is used to stain tissues. Eosin is a synthetic stain and attempts have been made to substitute eosin with a natural dye, one among them being rose extract. Staining using rose extract is economical, easily available and has not been explored before.
Aims and objectives: The study aims to compare between two different extraction methods for rose, namely maceration and soxhlet, to compare the staining efficacy of rose with (REM) and without mordant, to compare the efficacy of rose extracts with synthetic eosin, in both normal and pathological oral tissues [e.g., oral squamous cell carcinoma (OSCC)].
Materials and methods: Rose was dried, powdered, and extracted by maceration and soxhlet techniques. Tissue sections were stained with and without the mordant potassium alum. Hematoxylin and rose (H&R) and H&E stained normal and pathologic tissues were compared. Statistical analysis was done using Chi-square test.
Results: Statistically significant results were observed in sections stained by soxhlet method than that of maceration, sections stained by REM was comparatively better than those stained with rose extract alone, the normal and pathologic tissues stained with H&R gave comparable result with H&E.
Conclusion: The REM can be used as a substitute to eosin for the staining of tissues.
Clinical significance: Natural substitute for synthetic eosin.