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Practical Biochemistry for Medical Students
B Raghu
PART A: QUALITATIVE ANALYSIS
CHAPTER ONE:
REACTIONS OF CARBOHYDRATES
MOLISCH'S TEST
BENEDICT'S TEST
BARFOED'S TEST
FOULGER'S TEST
MUCIC ACID (GALACTOSACCHARIC ACID) TEST
TEST FOR SUCROSE
IODINE TEST
AMMONIUM SULPHATE PRECIPITATION
OSAZONE FORMATION
CHAPTER TWO:
REACTIONS OF PROTEINS
COLOR REACTIONS
Biuret Reaction
Aldehyde Reaction
Lead Acetate Test (Sulphur Test)
PRECIPITATION REACTIONS
REACTIONS OF ALBUMIN, PEPTONES, CASEINS AND GELATIN
Albumin
Globulin
Gelatin
Peptones (Secondary protein derivatives)
Casein
CHAPTER THREE:
URINE ANALYSIS
URINE COLLECTION
GENERAL CHARACTERISTICS
Volume
Color
Odor
Specific Gravity
pH
Turbid Urine
ABNORMAL CONSTITUENTS OF URINE
Proteins
Sugars
Blood
Bile Salts and Bile Pigments
Ketone Bodies in Urine
PART B: SPECIALISED TECHNIQUES AND INSTRUMENTATION
CHAPTER FOUR:
INTRODUCTION TO COMMONLY USED INSTRUMENTS
COLORIMETER
Introduction
THE PHOTOELECTRIC COLORIMETER
THEORY OF COLORIMETRY
Instructions for Operating the Instrument
FLAME PHOTOMETRY
The Flame Photometer
CHAPTER FIVE:
CHROMATOGRAPHY
PROCEDURE
CHAPTER SIX:
ELECTROPHORESIS
CHAPTER SEVEN:
AUTOANALYSER AND QUALITY CONTROL
PART C: CLINICAL CHEMISTRY
CHAPTER EIGHT:
BLOOD COLLECTION AND HANDLING, PREPARATION OF SERUM AND PLASMA FOR ANALYSIS
CHAPTER NINE:
PLASMA GLUCOSE ESTIMATION
INTRODUCTION
Principle
Procedure
Calculation
Result
CHAPTER TEN:
GLUCOSE TOLERANCE TEST
CASE-I
Laboratory Findings
Question
CHAPTER ELEVEN:
ESTIMATION OF SERUM TOTAL CHOLESTEROL
CASE-II
CHAPTER TWELVE:
SERUM UREA ESTIMATION
CHAPTER THIRTEEN:
SERUM CREATININE ESTIMATION AND THE CREATININE CLEARANCE TEST
INTRODUCTION
Clearance
Creatinine Clearance
ESTIMATION OF SERUM CREATININE
Principle
Procedure
Calculation of Results
CREATININE CLEARANCE TEST
Procedure
Estimation of Urine Creatinine
CASE-III
CHAPTER FOURTEEN:
SERUM TOTAL PROTEIN ESTIMATION
CHAPTER FIFTEEN:
SERUM ALBUMIN ESTIMATION AND THE A/G RATIO
CASE-IV
CHAPTER SIXTEEN:
ESTIMATION OF SERUM BILIRUBIN
INTRODUCTION
Principle
Procedure
Calculation
CHAPTER SEVENTEEN:
SERUM TRANSAMINASES [ALT (SGPT) AND AST (SGOT)]
CHAPTER EIGHTEEN:
SERUM ALKALINE PHOSPHATASE ESTIMATION
INTRODUCTION
Principle
Procedure
Calculation
CASE-V
CHAPTER NINETEEN:
SERUM URIC ACID ESTIMATION
CHAPTER TWENTY:
SERUM Na+ and K+ ESTIMATION
CASE-VI
CASE-VII
CASE-VIII
PART D: METABOLIC STUDIES
CHAPTER TWENTY ONE:
THE RESPIRATION OF MITOCHONDRIA AND OXIDATIVE PHOSPHORYLATION
THE OXYGEN ELECTRODE
Practice
Isolation of Mitochondria
The Respiration of Mitochondria and Oxidative Phosphorylation
Principle
Question
Answer
CHAPTER TWENTY TWO:
EFFECT OF INHIBITORS ON THE RESPIRATORY CHAIN
PART E: MOLECULAR BIOLOGY
CHAPTER TWENTY THREE:
MOLECULAR BIOLOGY
CHAPTER TWENTY FOUR:
THE ISOLATION OF DNA FROM PIG SPLEEN
METHOD
CHAPTER TWENTY FIVE:
THE ISOLATION OF RNA FROM YEAST
METHOD
CHAPTER TWENTY SIX:
THE ESTIMATION OF DNA BY THE DIPHENYLAMINE REACTION
METHOD
CHAPTER TWENTY SEVEN:
THE ESTIMATION OF RNA BY MEANS OF THE ORCINOL REACTION
METHOD
CHAPTER TWENTY EIGHT:
THE ISOLATION OF BACTERIAL DNA
METHOD
Extraction
First Ethanol Precipitation
RNase and Second Ethanol Precipitation
Isopropanol Precipitation
Assay for Nucleic Acids
UV Absorption of DNA
Quality of DNA
Solubility of DNA
CHAPTER TWENTY NINE:
THE ISOLATION AND CHARACTERIZATION OF EUKARYOTIC MRNA
METHOD
Isolation of Polyribosomal RNA
Column Preparations
Column Performance
Column Regeneration
Preparation of mRNA using the Oligo (dT) Columns
Precipitation of mRNA
APPENDIX
APPENDIX ONE:
NORMAL ADULT VALUES
APPENDIX TWO:
PROFILES
INDEX
TOC
Index
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