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Practical Biochemistry for Students
Varun Kumar Malhotra
1:
Methods of Expressing Concentration
2:
Physical Chemistry
3:
Carbohydrates
4:
Achromic Point
5:
Proteins
6:
Isoelectric Point
7:
Lipids
8:
Saponification Number
9:
Iodine Number
10:
Formal Titration
11:
Gastric Analysis
12:
Urine Analysis
13:
Food Analysis
14:
Collection and Preparation of Blood Specimen
COLLECTION AND PRESERVATION OF URINE SPECIMENS
Collection of Urine Specimens
Preservatives
Hydrochloric Acid
Toluene
Hibitane (Chlorhexidine diacetate)
Urine Analysis
Physical Tests
Specific Gravity
15:
Urinary Reducing Sugars
16:
Urinary Chlorides
17:
Urinary Creatinine
18:
Ascorbic Acid in Urine
19:
Serum Uric Acid
20:
Colorimetry
21:
Blood Sugar
22:
Glucose Tolerance Test (GTT)
23:
Blood Urea
24:
Urea Clearance
25:
Blood Cholesterol
26:
Serum Calcium
27:
Inorganic Phosphorus
28:
Serum Total Proteins and Albumin: Globulin Ratio
29:
Serum Bilirubin
30:
Prothrombin Time
31:
Liver Function Tests
32:
Demonstrations
ELECTROMETRIC DETERMINATION OF pH
Solution Tension and Electrode Potentials
Hydrogen Electrode
Calomel electrode
Glass Electrode
pH Meter
ESTIMATION OF NITROGEN CONTENT BY MICROKJELDAHL METHOD
Principle
Reaction
Procedure
a. Digestion
b. Distillation
c. Estimation
Reaction
Indicator
Calculations
Uses
FLAME PHOTOMETRY
Principle
1. Suction needle
2. Atomiser
3. Spray Chamber
4. Compressor
Procedure
Pathological Variations of Na+ and K+
ELECTROPHORESIS OF PLASMA PROTEINS
Apparatus
Media
Theory
Apparatus
Fixation
i. Thermal method
ii. Chemical method
Location
CHROMATOGRAPHY
Principle
1. Column Chromatography
2. Gas Chromatography
3. Partition Chromatography
4. Paper Chromatography
Descending Chromatography
Two-dimensional Chromatography
Isotopes
Radioactivity
Methods for Counting Radioactivity
Measurement of Radioctivity
Units of Radioactivity
Application of Isotopes
Hazards of Radioactivity
Precautions
CENTRIFUGE
Constructions
Operation
Precautions
pH METER
How to Operate
Precautions
ELISA
Principle
Types of ELISA
Appendix
INDEX
TOC
Index
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