A
Advantages of filtration 244
Adverse donor reactions 65
hematoma 67
management of hematomas 67
hyperventilation 69
moderate reactions 71
management 72
symptoms 72
nausea and vomiting 68
severe reactions 72
causes of convulsion or seizures 72
management 74
types of severe reactions 73
vasovagal reactions 69
Aplastic anemia 284
guidelines for transfusion therapy 285
platelet transfusion 286
red cell transfusion 285
treatment of aplastic anemia 284
Appropriate assays and testing strategies for transfusion transmitted infections 107
Arrangement of deep freezers and domestic refrigerators 33
blood bank refrigerator 33
deep freezers and domestic refrigerators 33
Arrangement of emergency seat 37
Arrangement of issuing counter 38
B
Biochemical changes in stored blood 32
Blood components 130
cryopoor plasma/single donor plasma 141
cryoprecipitate 138
fresh frozen plasma 132
platelet rich and platelet rich concentrate 135
red cell concentrate 130
washed red cells 142
Blood inventory from outside blood bank 42
Blood safety 204
C
Categories and proportions of errors 196
Classification of filters 243
Cleaning of blood bank equipment 22
blood bank refrigerator 22
deep freezer 23
plasma expresser 23
platelet agitator 24
refrigerated centrifuge 23
tube sealer 24
weighing balance 24
Cleaning of glassware and plastic 20
cleaning of microplates 21
cleaning of test tubes 20
automatic method 20
manual method 21
Cleaning of the premises of blood bank 21
Cleaning of the working table 22
Collection of blood for blood components 128
Component laboratory 123
Cryopreservation of blood 142
D
Deterrence/prevention of transfusion reactions 199
Difference between a blood bank refrigerator and a domestic refrigerator 34
Directed donations 51
Documentation at the issue counter 39
Donor room procedures 53
E
Equipment and consumables for component laboratory 124
Equipment and glassware for routine laboratory work 76
Equipment and glassware for special procedures laboratory 152
Essential requirements of blood transfusion services 18
Exchange transfusion 271
calculations for neonatal exchange transfusion 275
choice of blood group 272
indications 271
objectives 272
transfusion procedure 275
types 274
volume of blood 273
External quality control for HIV/HBS AG/HCV by indirect ELISA 111
G
General criteria for selecting apheresis donor 222
General requirements for apheresis 221
Guidelines for plasma transfusion 208
cryoprecipitate—depleted plasma 209
fresh frozen plasma 208
virus ‘inactivated’ plasma 209
Guidelines for platelet concentrates 209
random donor platelets 209
single donor platelets 212
single donor platelets vs random donor plates 213
Guidelines for red cell transfusion 207
Guidelines for whole blood transfusion 206
I
Infection markers screening laboratory: general instructions 109
Infectious markers laboratory 101
L
Laboratory studies 195
Leukocyte-depletion or leukodepletion 238
advantages 240
clinical conditions 240
comparison of percentage efficiency of leukoreduction and log reduction 240
leukodepletion levels 240
leukodepletion—AABB standards 239
methods of leukodepletion 241
buffy coat removal 242
centrifugation 241
freezing 242
leukoreduction by filtration 242
washing 241
M
Malaria 120
Management of transfusion reactions 183
Mandatory investigations for screening of blood 103
Monitoring the transfused patient 198
N
Neonatal transfusion 264
choice of blood group 269
guidelines 265
fresh frozen plasma transfution 267
granulocyte transfusion 268
platelet transfusion 267
red cell transfusion 266
indications 264
potential adverse reactions 270
pretransfusion testing for neonates and infants upto 4 months 269
Newer techniques in transfusion medicine 237
Nucleic acid amplification testing 252
P
Planning of blood transfusion services 7
environmental criterion 9
equipment criterion 12
for component laboratory 14
for routine laboratory equipment 12
required for a blood donation camp 16
required in blood donation room 15
required in infection markers screening laboratory 13
functional criterion 7
administrative and clerical offices 8
bleeding complex 7
blood component separation area 8
blood donation complex 7
blood storage area 8
laboratories 8
research/development/training area 8
service area 9
therapeutic area 7
work flow criterion 9
Platelet refractoriness 279
causes 279
diagnosis 280
corrected count increment 281
percent expected recovery 280
management 282
risk factors for developing platelet reactive antibodies 282
Post-donation counseling 63
Preparation of gum tape 26
Preparation of the working table 25
Prevalence of transfusion transmitted infections 106
Principle of apheresis 220
Principles of clinical transfusion practice 204
Problematic clinical situations 261
Procedure for cleaning of spills 26
Procedures carried out in the special procedures laboratory 155
autologous adsorption of warm reactive autoantibodies 170
cold antibodies/pre-warm procedure 164
confirmation of weak A or B subgroup by adsorption and elution 160
determination of ABH secretor status 158
dissociation of IgG from RBC surface by chloroquine 168
ether/heat elution procedure 172
expanded ABH panel for investigation of ABO discrepancy 156
freezing and thawin of reagent cells 166
identification of RBC antibodies 162
investigation of polyagglutinable RBCs 173
platelet immunofluorescence test 174
Procedures in the serology laboratory 77
ABO and Rh by microplate technique 82
determining working dilution of antisera 83
interpretation 86
procedure 83
reagents and materials 82
ABO compatibility test by microplate technique 86
aim 86
reagents and materials 86
ABO grouping by tube technique 77
grading of agglutination 79
preparation of reagent cells 78
procedure 78
reagents and materials 77
antibody screening for atypical antibodies by tube technique 90
aim 90
methods 90
reagents and materials 91
controls 91
procedure 91
antibody screening for atypical antibodies by microplate technique 95
direct antiglobulin test (DAT) 99
cross-match by LISS and enzyme AHG tube technique 88
aim 88
interpretation 87
procedure 87
reagents and materials 88
enzyme two-stage method 90
LISS 88
rhesus typing by tube technique 80
interpretation 81
procedure 80
reagents and materials 80
Protocol for ordering blood 204
S
Serology laboratory 75
Staffing for blood bank (personnel) 18
Storage and issue of blood 31
Storage and issue of blood in the ward 42
Storage of fresh frozen plasma in the deep freezer 36
Storage of platelet concentrate in the platelet agitator 35
Storage of whole blood and packed cells in the blood bank refrigerator 34
Storage protocols of the various blood components 32
T
The appropriate clinical use of blood and blood products 202
Thrombocytopenia in pregnancy 277
guidelines for transfusion therapy in ITP 277
idiopathic thrombocytopenic purpura in pregnancy 277
thrombotic thrombocytopenic purpura in pregnancy 278
Transfusion in solid organ transplantation 297
transfusion protocols for 298
choice of blood groups 298
dosage of blood components in 299
general considerations 300
Transfusion transmitted infections 104
Transportation 129
Trouble shooting for microplate ELISA 115
Trouble-shooting during the apheresis procedure 224
Types of filtration 244
Types of transfusion reactions 184