Practical Biotechnology P Ramadass, A Wilson Aruni
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1Practical Biotechnology
P Ramadass MVSc DVPH PhD (NZ) Professor and Head Department of Animal Biotechnology Madras Veterinary College Chennai Tamil Nadu A Wilson Aruni BSc MVSc PhD Associate Professor Centre and ARIS Cell Bioinformatics Madras Veterinary College Chennai Tamil Nadu
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Practical Biotechnology
© 2007, P Ramadass, A Wilson Aruni
All rights reserved. No part of this publication should be reproduced, stored in a retrieval system, or transmitted in any form or by any means: electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the authors and the publisher.
This book has been published in good faith that the material provided by authors is original. Every effort is made to ensure accuracy of material, but the publisher, printer and authors will not be held responsible for any inadvertent error(s). In case of any dispute, all legal matters are to be settled under Delhi jurisdiction only.
First Edition: 2007
9788184480795
Typeset at JPBMP typesetting unit
Printed at Paras Press
3Preface
Biotechnology has risen up to become the pinnacle of all biological sciences owing to its vast applicability in various fields of science and technology. Modern biotechnology has played a substantial role in the development of the health care and chemical industries. It has made possible the availability of several diagnostic, prophylactic and therapeutic products. Recombinant DNA technology and genetic engineering techniques find several applications in the areas of vaccines, foods, antibiotics, alcohols, hormones and monoclonal antibodies. It has been stated that next to computer science, biotechnology stands a rewarding career-oriented manpower.
In recent times, intensive research is being taken up in this discipline as many undergraduate and postgraduate courses are being offered in many colleges. Though more theoretical knowledge is imparted to the students of biotechnology in different colleges, the practical knowledge which could be given to the students is lacking. The reason for these lacunae could be either due to the nonavailability of laboratory equipments and technical manpower in such institutions. A comprehensive textbook covering different biotechnological methods, that could cater to the need of students is lacking now.
The quest of such lacunae is satiated by this textbook Practical Biotechnology, which brings about the practical applications and protocols on areas of applied biotechnology and molecular biology, viz., cell culture, hybridoma technology, nucleic acid isolation and electrophoresis, nucleic acid probes and hybridization methods, PCR and related techniques and nucleic acid sequencing methods. There are seven chapters in this book that covers all the above mentioned topics in detail, elaborating the principles and step-by-step protocols.
The authors have extensive experience in teaching and research in Biotechnology and have also authored Textbook of Animal Biotechnology, which has gone through couple of editions. Though the protocols given are standardized to the authors' laboratory, the authors do not claim them as 4their original contribution, because they are the compilation of methods from different sources. A number of standard textbooks, journals and review papers were consulted to make this book an authenticated and standard practical biotechnology manual. The students would be able to carry out different molecular methods using this practical book with ease.
The authors express their apologies for any mistakes inadvertently committed in preparation of this textbook. Any suggestions from the readers in improving the book in further editions are most welcome.
P Ramadass
A Wilson Aruni