Shivaraja Shankara YM Associate Professor Department of Biochemistry KVG Medical College
Sullia, Karnataka
Co-Authors Ganesh MK Asst. Professor Dept. of Biochemistry KVG Dental College
Sullia, Karnataka
Hemavathi A Asst. Professor Dept. of Biochemistry KVG Medical College
Sullia, Karnataka
Jitendar P Vij
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Laboratory Manual for Practical Biochemistry
© 2008, Jaypee Brothers Medical Publishers
All rights reserved. No part of this publication should be reproduced, stored in a retrieval system, or transmitted in any form or by any means: electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the authors and the publisher.
First Edition: 2008
9788184482591
Typeset at JPBMP typesetting unit
Printed at Rajkamal
FM4
Respectfully Dedicated To
Dr Kurunji Venkatramana Gowda
Founder President
Academy of Liberal Education
Sullia
FM5Preface
Knowledge of practical aspects of biochemistry is essential for a medical student to understand diagnostic and prognostic status of any ailment. We have always felt the need for a complete and comprehensive book on orientation towards various aspects of practical biochemistry. This book is written in simple language, covering the practical syllabus for MBBS, BDS, BSc MLT and BSc nursing students as per the curriculum and guidelines of Rajiv Gandhi University of Health Sciences, Bangalore and most of other Health Universities in India. The book includes both theory and practical aspects of clinical biochemistry. A clear-cut practical concept has been offered in the book in respect of all qualitative and quantitative experiments. Chapters on Demonstration Experiments such as colorimetry, electrophoresis, chromatography, etc. are also included. Questions at the end of each chapters and normal values of various biochemical parameters in blood and urine are also included in this book.
We hope that this book will meet the requirement of undergraduate students studying biochemistry. We will be glad to accept constructive criticism and suggestions from the faculty, students and readers to make this book a better one in subsequent prints.
Shivaraja Shankara YM
Ganesh MK
Hemavathi A
FM6Acknowledgements
It is our privilege to express our sincere gratitude to Dr KV Chidananda, Vice President and Dr KV Renuka Prasad, General Secretary, AOLE for their constant encouragement and support. We are grateful to Dr VK Jayapalan, Director, Dr KG Nayak, Dean Academics, Dr Sheela G Nayak, Principal, KVG Medical College and Hospital, Sullia for their constant inspiration and encouragement. We also express our sincere thanks to Mrs Shobha Chidananda, Treasurer, AOLE, Dr Jyothi R Prasad, Director AOLE and Prof. AS Ramakrishna, Administrator, AOLE Group of Institutions for their help and moral support. We gratefully acknowledge the cooperation of Dr Cariappa, Asst. Prof., Department of Biochemistry and Mr Monappa and his office staff in KVG Medical College.
We express our immense gratitude to Dr Shivashankara AR, Associate Professor of Biochemistry, SDM Institute of Medical Sciences, Dharwad for his timely help and valuable suggestions.
We are thankful to Mr Tarun Duneja, General Manager (Publishing) and Mr K Suresh Kumar, Sales Manager, Jaypee Brothers Medical Publishers (P) Ltd, New Delhi, for their sincere effort and cooperation in bringing out this book in time.