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Illustrated Medical Microbiology
Satish Gupte
Introduction
SECTION - 1: BACTERIOLOGY
General Bacteriology
BACTERIA
Shape of Bacteria
Cell Wall
Cytoplasmic Membrane
Cytoplasm Contains
RIBOSOMES (Ribonucleoproteins)
Polysome
Mesosome
Intracytoplasmic Inclusions
Nucleus
Flagellum
Fimbriae (Pili)
Functions
Bacterial Spore
Sporulation Process
The shape and position of the spore in bacterial cell
Staining of Bacteria
Ziehl Neelsen Stain
CULTURE TECHNIQUES
Description of Growth in Liquid Media
STERILIZATION
Methods of Sterilization
Heat
Flaming
MOIST HEAT
Vaccine Bath
Inspissator
Two types of sterilizers available
Autoclave
Filtration: Sterilization of
Radiation
Anaerobiasis
IDENTIFICATION OF BACTERIA
Microscopic Examination: Gives information
After Gram staining we get information like
Resistance
Culture Character
Biochemical Reactions
ANTIGENIC ANALYSIS
BACTERIOPHAGE TYPING
PATHOGENICITY IN LABORATORY ANIMALS
Route of Inoculation
RESISTANCE TO ANTIBIOTICS
Sensitivity pattern useful in
NUTRITIONAL AND OTHER REQUIREMENTS OF BACTERIA
Minerals (required in traces)
Gas
Obligate Anaerobes
Carbon dioxide
Moisture
Growth Factors
Temperature
pH : Optimum pH 7.4
Osmotic Pressure
Light
Vibrations
Growth Curve
Continuous Culture
ANTIMICROBIAL AGENTS
Mode of Action
Mechanism of Action
INFECTION
Classification of Infection
Methods of Transmission
Insect
Congenital
Specifically Neutralized by Antitoxin
Enzymatic Action
Specific Affinity for Tissue
Endotoxin
OTHER BACTERIAL PRODUCTS
Route of Infection
SECTION - 2: BACTERIAL GENETICS
Bacterial Genetics
GENE THERAPY
Aim
Indications
Techniques
Problems anticipated with this treatment
SECTION - 3: IMMUNOLOGY
Immunology
LYMPHOCYTES
Properties
Plasma cell
Properties
Human K Cell
Natural Killer Cell
Multiple Antigens
Example of Adjuvants
Immunosuppressive Agents
Theories of Antibody Synthesis
Examples
Cytolytic and Cytotoxic Reactions (Type 2)
Examples
Mechanisms of Arthus Reaction
Type IV Hypersensitivity (Delayed Type)
Examples
Type V Hypersensitivity (Stimulatory)
Prevention of Graft Rejection
AUTOIMMUNE DISEASES
IMMUNOLOGICAL SURVEILLANCE
IMMUNOLOGY OF MALIGNANCY
TUMOR ASSOCIATED ANTIGENS (4 TYPES)
SECTION - 4: VIROLOGY
General Virology
CULTURE OF VIRUS
Oncogenic RNA Viruses
Avian Oncoviruses
SECTION - 5: PARASITOLOGY
Protozoology
CHARACTERS OF TRICHOMONAS
LEISHMANIA DONOVANI
Geographical Distribution
Amastigote (LD body)
Promastigote
Pathogenesis and Pathology
Spleen
Microscopic
Bone marrow
Prophylaxis
Treatment
TRYPANOSOMA CRUZI
Morphology
Diagnosis
Prophylaxis
Morphology
Developing trophozoites
Immature Schizont
Mature Schizont
Microgametes
Macrogametocytes
Pathology
Clinical Picture
Diagnosis
SECTION - 6: MYCOLOGY
General Mycology
MYCOLOGY (STUDY OF FUNGI)
CLASSIFICATION OF FUNGI
Systemic Classification (on the basis of spore formation)
Phycomycetes
Ascomycetes
Basidiomycetes
Fungi Imperfecta
Superficial Mycology
DERMATOPHYTES (3 GENERA)
Microsporum (Hair and Skin)
Trichophyton (Skin, Hair and Nails)
Culture
Treatment
TRICHOSPORON CUTANEUM
Morphology
Laboratory Diagnosis
Treatment
PIEDRA HORTAI
Culture
Clinical Features (Black piedra)
Laboratory Diagnosis
Treatment
Laboratory Diagnosis
Treatment
PITYROSPORUM ORBICULTURE (MALASSEZNIA FURFUR)
Morphology
Culture
Clinical Features
Produces Disease called Pityriasis Versicolor
Laboratory Diagnosis
Treatment
Subcutaneous Mycology
SPOROTHRIX SCHENCKII
Morphology (Dimorphic)
Microscopic Appearance
Microscopic appearance
Clinical Features
Laboratory Diagnosis
Laboratory Diagnosis
Treatment
MYCETOMA
Etiology
Laboratory Diagnosis
Clinical Features
Treatment
Systemic Mycology
SYSTEMIC MYCOSIS CAUSING FUNGI CHROMOMYCOSIS
Etiology
Clinical Features
Laboratory Diagnosis
Treatment
CRYPTOCOCCUS NEOFORMANS
Morphology (microscopic)
Treatment
HISTOPLASMA CAPSULATUM
Morphology (Dimorphic)
Culture
Colony
Clinical Features
Laboratory Diagnosis
Treatment
BLASTOMYCES DERMATITIDES
Morphology (Dimorphic)
Pathogenesis
Clinical Features (North American Blastomycosis)
Treatment
PARACOCCIDIODES BRASILIENSIS
Morphology (Dimorphic)
At Room Temperature
Clinical Features (South American Blastomycosis)
Laboratory Diagnosis
Treatment
COCCIDIOIDES IMMITIS
Morphology
Clinical Picture (San Joaquin Valley Fever)
Laboratory Diagnosis
Treatment
ASPERGILLUS FUMIGATUS
Morphology
Culture
Microscopic appearance
Clinical Features
Laboratory Diagnosis
Treatment
MUCOR
Morphology
Culture
Clinical Picture
Laboratory Diagnosis
Treatment
RHIZOPUS
Morphology
Culture
ABSIDIA
Culture
Microscopic Appearance
Clinical Features
Laboratory Diagnosis
Treatment
PENICILLIUM
Morphology
Culture
Rapid growth
Microscopic
INDEX
TOC
Index
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