An Easy Guide for Practical Biochemistry Divya Shanthi D’sa, Sowbhagya Lakshmi
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1An Easy Guide for Practical Biochemistry
2An Easy Guide for Practical Biochemistry
Divya Shanthi D'Sa MBBS, DFH Lecturer Department of Biochemistry Shimoga Institute of Medical Sciences Shimoga, Karnataka, India Sowbhagya Lakshmi MSc, PhD Professor of Biochemistry Shimoga Institute of Medical Sciences Shimoga, Karnataka, India
3Published by
Jitendar P Vij
Jaypee Brothers Medical Publishers (P) Ltd
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An Easy Guide for Practical Biochemistry
© 2010, Jaypee Brothers Medical Publishers
All rights reserved. No part of this publication should be reproduced, stored in a retrieval system, or transmitted in any form or by any means: electronic, mechanical, photocopying, recording, or otherwise, without the prior written permission of the authors and the publisher.
First Edition: 2010
9788184487930
Typeset at JPBMP typesetting unit
Printed at Ajanta Offset & Packagins Ltd., New Delhi
4Dedicated to
Students
5Preface
Biochemistry, a fascinating subject that deals with every function and every reaction of the body. Clinical biochemistry plays a tremendous impact on the diagnosis and treatment of patients. Medical students should be aware of the practicals, diagnostic parameters and their estimations. They should acquire sound knowledge about the diagnostic reports and its implications which aids in diagnosis and prognosis of the disease.
Biochemistry is the most fast growing subject, extensively applicable to understand the disease at molecular level. Estimations of various biochemical parameters definitely give an insight to understand the normal metabolism and its aberrations leading to diseases, which forms the foundation for medicine.
Biochemistry should be encouraged in relation to health and disease which will make the subject more interesting and fascinating to the students. We are hopeful that this practical biochemistry book will help the medical students to envisage about the various facts encountered in the reactions in the body.
Dear students let us admit that ‘Biochemistry’ is rarely a medical graduates' favorite given the fact that it is non- ‘Clinical’, extensive, volatile and there is little in it to arouse any amount of interest in the medical graduates. In our teaching biochemistry to undergraduate medical students we have realized practicals is where they have shown some amount of enthusiasm towards biochemistry.6
Our main aim is to make this book simple and attractive to the undergraduate medical students as far as possible which is apparent from the title of the book. Towards achieving this goal, each practical session has been reorganized such that it becomes easy to understand. Wherever possible, the subject is presented in tabular format such that it becomes very concise; and all test results are given in color such that the simple task of reading the book itself realizes one doing the practicals. Fundamental concepts and principles behind each experiment are explained in a simple way.
Our only genuine concern is to help you to understand the subject in an easy and organized way such that this little knowledge comes as a big help not only in your exams but also in your future medical career.
We will be glad to accept constructive criticism and fruitful suggestion to make this book a better one.
Divya Santi D'Sa
Sowbhagya Lakshmi
7Acknowledgments
The authors are indebted to student community who constantly motivate and make us stay updated with the latest knowledge.
It is our proud privilege to express our gratitude to our colleagues Dr Govindaswamy, Dr I C Vinay, Dr Jyothi R S and Mr Anil Kumar Suryavamshi involved in reviewing the manuscript of this book.
I take this opportunity to thank computer operators Mrs Veena Jayaram and Mr Sunder for their help in preparing the manuscript. We are also very grateful to our lab technicians Mr Vasant, Mr Shankar, Mr Girish and Miss Rajeshwari for their valuable assistance.
The authors are indebted to Jaypee Brothers Medical Publishers (P) Ltd., New Delhi, for bringing our effort to a proper shape in a way that it could become a good pocket companion to the students.
We are very grateful to Shri Jitendar P Vij, Chairman and Managing Director and publishing team of M/s Jaypee Brothers Medical Publishers (P) Ltd., for their sincere efforts and cooperation.