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The Short Textbook of Medical Microbiology (Including Parasitology)
Satish Gupte
PART I: GENERAL BACTERIOLOGY
1:
Glossary of Microbiology
2:
History and Scope
INTRODUCTION
Historical Events
MICROBIOLOGY
APPLICATION TO HEALTH
Scope of Microbiology
Free Living Nature of Bacteria
BIOLOGICAL WEAPONS
Molecular Epidemiology
Epidemic Prone Diseases
Ancient
Middle Era (killer diseases)
Middle Era (emerging/reemerging)
Current Era
Threat of Rare Diseases
Application of Molecular Epidemiology
3:
Morphology of Bacteria
SLIME LAYER
CAPSULE (FIG. 3.4)
Demonstration of Capsule
Functions
CELL WALL
Chemical Structure of Cell Wall
Gram-positive cell wall:
Demonstration
Functions
CYTOPLASMIC MEMBRANE
Demonstration
Functions
CYTOPLASM
Functions
Intracytoplasmic Inclusions
NUCLEUS
FLAGELLA
Demonstration
FIMBRIAE
Functions
SPORES (FIG. 3.12)
Formation of Spores (Fig. 3.13)
Germination of Spore
Study of Morphology of Bacteria
Optical Methods
UNSTAINED PREPARATIONS
STAINING OF BACTERIA
GRAM's STAIN
ACIDIC ACRIDINE ORANGE STAIN
ALBERT STAIN
ZIEHL-NEELSEN STAIN
SPORE STAIN
CAPSULAR STAIN
4:
Nutritional Requirements of Bacteria
GROWTH REQUIREMENTS
Nutritional Requirements
Gas Requirements
Moisture
Accessory Nutritional Requirements
GROWTH CURVE
Lag Phase
Log Phase
Stationary Phase
Decline Phase
Survival Phase
CONTINUOUS CULTURE
Factors Influencing Growth
BACTERIAL REPRODUCTION
GENERATION TIME
SYNCHRONOUS GROWTH
BACTERIAL COUNT
5:
Bacterial Metabolism
BACTERIAL METABOLISM
Catabolism
Redox Potential
Anabolism
METABOLISM OF NITROGENOUS COMPOUNDS
6:
Media for Bacterial Growth
FLUID MEDIA
Types of Liquid Media
Broth
Peptone
SOLID MEDIA
Classification of Media
Culture Techniques
Methods of Culture
METHODS OF ANAEROBIC CULTURE
7:
Classification and Identification of Bacteria
DEVELOPMENT OF RAPID METHODS FOR IDENTIFICATION OF ORGANISMS
8:
Sterilization and Disinfection
APPLICATION OF DISINFECTANTS
In Bacteriology
In Surgical Procedures
In Hospitals
In Public Health Services
Physical
Chemicals
Physical Methods
Dry Heat
Precautions in Use of Hot Air Oven
Sterilization Control of Hot Air Oven
Moist Heat
Temperature below 100°C
Temperature at 100°C
Temperature above 100°C
AUTOCLAVE (FIG. 8.3)
Types of Autoclave
Sterilization by Filtration
Other Uses of Filter
Radiations
Ultrasonic and Sonic Vibrations
Chemical Methods
Uses of Disinfectant
EVALUATION TESTS OF DISINFECTANT
9:
Infection
10:
Antimicrobial Therapy
PART II: BACTERIAL GENETICS
11:
Bacterial Genetics
LANDMARKS IN GENETICS
STRUCTURE OF DNA
Chromosome Function
PLASMID
Features of Bacterial Plasmids
Molecular Classes of Plasmid
Small Plasmids
Large Plasmids
EPISOMES
GENOTYPIC AND PHENOTYPIC VARIATION
MUTATION
Types of Mutation
Point Mutation
Multisite Mutation
Effects of Mutation
AMES TEST
TRANSMISSION OF GENETIC MATERIAL
Transformation
Transduction
Lysogenic Conversion
Conjugation (Fig. 11.5)
Protoplast Fusion
APPLIED BACTERIAL GENETICS
Genetic Engineering (Fig. 11.6)
Techniques Established in Genetic Engineering
PROBE
Application of Nucleic Acid Probes
Polymerase Chain Reactions (PCR)
Blotting Technique
Immunoblotting
Gene Therapy
Techniques
Future Prospects
Nobel Prize in Medicine—1993
Nobel Prize in Medicine—2006
PART III: IMMUNOLOGY
12:
Immunity
HISTORICAL EVENTS IN IMMUNOLOGY
APPLICATION OF IMMUNOLOGY
IMMUNITY
INNATE IMMUNITY
Mechanism of Innate Immunity
Humoral Factors
Acute Phase Proteins
Cellular Factors
ACQUIRED IMMUNITY
VACCINATION
History of Development of Immunization
NEWER VACCINES
Advantages of Newer Vaccines
Disadvantages of Newer Vaccines
APPLICATION OF VACCINE
13:
Antigen
PROPERTIES OF ANTIGEN
SUPERANTIGEN
14:
Antibodies—Immunoglobulins
IMMUNOGLOBULINS
Structure of Immunoglobulin
Papain Digestion (Fig. 14.1)
Pepsin Digestion (Fig.14.2)
Immunoglobulin Domains (Fig. 14.5)
Immunoglobulin Antigen Determinants
Function of Immunoglobulins
Immunoglobulin Classes (Table 14.1)
Immunoglobulin Class (Isotype Switching)
15:
Antigen and Antibody Reaction
PRECIPITATION
Uses of Precipitation Reaction
LATTICE HYPOTHESIS (FIG. 15.1)
Techniques of Precipitation Reaction
AGGLUTINATION REACTIONS
Uses
TECHNIQUE OF AGGLUTINATION TEST
Direct Agglutination Test
Indirect or Passive Agglutination Test
Neutralization Test
Opsonization
Immune Adherence Test (Treponema pallidum)
Immunofluorescence
Immunoelectron Microscopic Test
16:
The Complement System
CONGLUTININ
Complement Dependent Serological Tests
17:
Structure and Functions of Immune System
ORGANS PRODUCING ANTIBODY
CENTRAL LYMPHOID SYSTEM
NEW CLASSIFICATION OF T CELLS (TABLE 17.1)
Peripheral Lymphoid System
Lymph Node (Fig. 17.1)
Spleen (Fig. 17.2)
Cells of Lymphoreticular System
Cellular Basis of Two Components of Immunity
18:
The Immune Response
HUMORAL IMMUNE RESPONSE
Primary and Secondary Responses
Primary Response (Fig. 18.1)
Secondary Response (Fig.18.2)
FACTORS INFLUENCING ANTIBODY PRODUCTION
THEORIES OF ANTIBODY PRODUCTION
CELLULAR IMMUNE RESPONSE
CYTOKINES
LYMPHOKINES
Affecting Macrophages
Affecting Lymphocytes
Affecting Granulocytes
Affecting Cultured Cell
Producing Effects in vivo
INTERLEUKINS
DETECTION OF CMI
SCOPE OF CELL MEDIATED IMMUNITY
19:
Hypersensitivity
HYPERSENSITIVITY
Classification
TYPE I REACTIONS
Anaphylaxis
Passive Cutaneous Anaphylaxis
Schütz-Dale Phenomenon
Mechanism of Anaphylaxis
Pharmacological Mediators of Anaphylaxis (Table 19.3)
ATOPY
TYPE II REACTIONS
Cytotoxic and Cytolytic
TYPE III REACTIONS
Immune Complex Diseases
Arthus Reaction
Serum Sickness
DELAYED HYPERSENSITIVITY (TYPE IV REACTION)
Manifestations
CLASSICAL TYPE
GRANULOMATOUS REACTIONS
Type V or Stimulation Hypersensitivity
SCHWARTZMAN REACTION
20:
Immunohematology
21:
Miscellaneous
IMMUNE DEFICIENCY SYNDROME
ACQUIRED IMMUNE DEFICIENCY SYNDROME (AIDS)
AUTOIMMUNE DISEASES
Mechanism of Autoimmune Disease
FEATURES OBSERVED IN AUTOIMMUNE DISEASES
IMMUNOLOGY OF TRANSPLANTATION
IMMUNOLOGICAL SURVEILLANCE
IMMUNOLOGICAL TOLERANCE
HISTOCOMPATIBILITY ANTIGEN (HLA antigen)
MHC Restriction
LIMULUS AMEBOCYTE LYSATE TEST (LAL TEST)
RADIOIMMUNOASSAY METHOD
Immunoblotting
ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)
MONOCLONAL ANTIBODIES
NOBEL PRIZE IN MEDICINE 1987
Generation of Immunoglobulin Diversity
PART IV: SYSTEMATIC BACTERIOLOGY
22:
Staphylococcus
MICROCOCCACEAE
Classification
STAPHYLOCOCCUS AUREUS (Staphylococcus pyogenes)
Selective Media
Factors Influencing Pigment Production
Biochemical Reactions
Characteristics of Pathogenic Strain
BIOTYPES
Enzymes Produced
Toxins
Other Toxins
Virulence Factors
Laboratory Diagnosis
SEROLOGICAL METHODS FOR ASSAY OF STAPHYLOCOCCUS AUREUS ENTEROTOXIN
METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS
STAPHYLOCOCCUS SAPROPHYTICUS
STAPHYLOCOCCUS ALBUS
Cultural Characters
STAPHYLOCOCCUS CITRUS
MICROCOCCUS TETRAGEN (GAFFKYA TETRAGENA)
Cultural Character
SARCINA
23:
Streptococcus
GROUP A STREPTOCOCCUS PYOGENES
Toxin Production
Laboratory Diagnosis
GROUP B
GROUP C
GROUP D
Cultural Characters
ALPHA HEMOLYTIC STREPTOCOCCI
ANAEROBIC STREPTOCOCCUS
24:
Pneumococcus
25:
Neisseria
NEISSERIA MENINGITIDIS
Laboratory Diagnosis
NEISSERIA GONORRHOEAE
Laboratory Diagnosis
Commensal Neisseriae
Non-gonococcal Urethritis
Etiology
Veillonellae
26:
Corynebacterium
CORYNEBACTERIUM DIPHTHERIAE
Biochemical Reactions
Resistance
Antigenic Structure
Bacteriophage Typing
Toxin
Mode of Action of Toxin
Pathogenicity
Complications
Laboratory Diagnosis
Bacteriological Investigations
Virulence Test
Immunity
Treatment
DIPHTHEROIDS
27:
Mycobacteria
MYCOBACTERIUM TUBERCULOSIS
Culture Characters
Resistance
Determinants of Mycobacterium Tuberculosis Pathology
Laboratory Diagnosis (Table 27.2)
Concentration Methods
Newer Drug Screening Methods of Mycobacterium Tuberculosis
IMMUNOLOGICAL INVESTIGATIONS
Adenosine Deaminase Activity (ADA)
Tuberculin
False Negative Tuberculin Test
BCG (Bacille Calmette-Guérin)
Complications of BCG
Local
Regional
General
ATYPICAL MYCOBACTERIA (Table 27.3)
SKIN PATHOGENS
MYCOBACTERIUM LEPRAE
Laboratory Diagnosis
MYCOBACTERIUM LEPRAE MURIUM
28:
Bacillus
BACILLUS ANTHRAX
Biochemical Reactions
Laboratory Diagnosis
BACILLUS SUBTILUS
BACILLUS CEREUS
29:
Clostridium
CLOSTRIDIUM TETANI
Laboratory Diagnosis
CLOSTRIDIUM PERFRINGENS (Clostridium welchii)
NAGLER's REACTION
Laboratory Diagnosis
Microscopic Examination
Animal Pathogenicity
Bacteriological Diagnosis of Clostridium perfringens Food Poisoning
CLOSTRIDIUM BOTULINUM
Laboratory Diagnosis
Bacteriological Investigations
CLOSTRIDIUM DIFFICILE
Morphology
Toxins
Pathogenesis
Laboratory diagnosis
Treatment
30:
Enterobacteriaceae
ESCHERICHIA COLI
Pathogenesis
Laboratory Diagnosis
Smear Examination
Culture
EDWARDSIELLA
CITROBACTER
KLEBSIELLA
ENTEROBACTER
HAFNIA
SERRATIA
PROTEUS
Laboratory Diagnosis
SHIGELLA
Biochemical Reactions (Fig. 30.3)
TOXIN
Laboratory Diagnosis
SALMONELLA
Antigenic Variation
Laboratory Diagnosis
Interpretation of Widal Test
31:
Pseudomonas
PSEUDOMONAS AERUGINOSA (Pseudomonas pyocyanea)
Antigenic Structure
TOXINS AND ENZYMES PRODUCED BY PSEUDOMONAS
Laboratory Diagnosis
32:
Vibrio
VIBRIO CHOLERAE
Special Media
Laboratory Diagnosis
Hematological Investigations
Bacteriological Investigations
Vibrio El Tor
Vibrio Cholerae 139
Non-agglutinable Vibrios (NAV)
Aeromonas and Plesiomonas
33:
Campylobacter
MORPHOLOGY
BIOCHEMICAL REACTIONS
PATHOGENESIS
CLINICAL FEATURES
LABORATORY DIAGNOSIS
TREATMENT
CAMPYLOBACTER JEJUNI MORPHOLOGY
CULTURE
BIOCHEMICAL CHARACTERS
PATHOGENESIS
CLINICAL FEATURES
LABORATORY DIAGNOSIS
Treatment
34:
Helicobacter Pyloridis
MORPHOLOGY
CULTURE
BLOOD AGAR MEDIUM
BIOCHEMICAL REACTIONS
PATHOGENESIS AND CLINICAL FEATURES
TOXINS AND ENZYMES PRODUCTION
LABORATORY DIAGNOSIS
Histology
Culture
Biopsy Urease Test
Urea Breath Test
Serology
TREATMENT
NOBEL PRIZE IN MEDICINE (2005)
35:
Brucella
36:
Pasteurella, Yersinia and Francisella
37:
Hemophilus
38:
Bordetella
BORDETELLA PERTUSSIS
Complications
Laboratory Diagnosis
Hematological Investigations
Bacteriological Investigations
Serological Investigations
BORDETELLA PARAPERTUSSIS
BORDETELLA BRONCHISEPTICA
39:
Spirochaetes
BORRELIA
Laboratory Diagnosis
Bacteriological Investigations
Borrelia Vincentii
LYME DISEASE
LEPTOSPIRA
Pathogenicity
Laboratory Diagnosis
Hematological Investigations
Bacteriological Investigations
Serological Test
TREPONEMA
Pathogenicity
Laboratory Diagnosis
Bacteriological Investigations
Secondary Stage
Tests using Live T. Pallidum
Test using Killed T. Pallidum
Test using T. Pallidum Extract
Treponema Pertenue
40:
Miscellaneous Bacteria
MYCOPLASMATACEAE
MYCOPLASMA
MYCOPLASMA PNEUMONIAE(EATON'S AGENT)
Laboratory Diagnosis
ACTINOMYCETES
ACTINOMYCES
Laboratory Diagnosis
NOCARDIA
ACTINOMYCOTIC MYCETOMA
LISTERIA MONOCYTOGENES
Moraxella Catarrhalis
ERYSIPELOTHRIX INSIDIOSA
STREPTOBACILLUS MONILIFORMIS
SPIRILLUM MINUS
DONOVANIA GRANULOMATIS
LACTOBACILLI
MOBILUNCUS
HELICOBACTER PYLORI
CAT SCRATCH DISEASE
ANAEROBIC GRAM-NEGATIVE BACILLI
Bacteroides
Fusobacterium
Dialister
Spherophorous
Laboratory Diagnosis
41:
Rickettsiae
TYPHUS FEVER GROUP
SPOTTED FEVER
GENUS ORIENTIA SCRUB TYPHUS
EHRLICHIA
GENUS COXIELLA Q FEVER
TRENCH FEVER
Laboratory Diagnosis of Rickettsial Diseases
Hematological Investigations
Bacteriological Investigations
Serology
Treatment
BARTONELLA BACILLIFORMIS
42:
Chlamydiae
LYMPHOGRANULOMA VENEREUM
Laboratory Diagnosis
TRACHOMA AND INCLUSION CONJUNCTIVITIS
Laboratory Diagnosis
Inclusion Conjunctivitis
Laboratory Diagnosis
PSITTACOSIS
Laboratory Diagnosis
Serological Diagnosis
43:
Newer Bacteria
LEGIONELLA PNEUMOPHILA
CLOSTRIDIUM DIFFICILE
KINGELLA KINGAE
CARDIOBACTERIUM HOMINIS
DF-2 BACTERIA
GARDNERELLA VAGINALIS
YERSINIA ENTEROCOLITICA
FLESH EATING BACTERIA
BORRELIA BURGDORFERI
44:
Microbiology of Oral Cavity
PART V: VIROLOGY
45:
General Characteristics of Viruses
INTERACTIONS AMONG VIRUSES
Cultivation Viruses
Disadvantages of Egg Inoculation
INCLUSION BODIES
LABORATORY DIAGNOSIS
Microscopic Examination
Identification in Cell Culture
Detection of Viral Antigens
Detection of Viral Nucleic Acids
Serology
46:
Chemotherapy of Viral Diseases
INTERFERON
Synthetic Polynucleotides and Other Inferferon Inducers
Poly I and Poly C
Current Research
ANTIVIRAL AGENTS
AGENTS INHIBITING REVERSE TRANSCRIPTASE
BROAD SPECTRUM NUCLEOSIDE ANALOGUES
NOBEL PRIZE IN MEDICINE-1988
47:
Classification of Viruses
48:
Oncogenic Viruses
MECHANISM OF VIRAL ONCOGENESIS
ONCOGENE VIROGENE HYPOTHESIS
Possible Viral Causation of Human Cancer
NOBEL PRIZE 1989 MEDICINE
49:
DNA Viruses
POXVIRUS
Morphology
Antigenic Properties
SMALLPOX (VARIOLA MAJOR)
Pathogenesis
Clinical Features
Laboratory Diagnosis
Collection of Specimen
Demonstration of Virus
Demonstration of Antibody
Prophylaxis
Vaccination
Method of Vaccination
Response of Vaccination
Complication of Primary Vaccination
MOLLUSCUM CONTAGIOSUM
ADENOVIRUSES
Herpes Virus (Fig. 49.4)
Human Herpes Virus Type 6
Human Herpes Virus Type 7
Human Herpes Virus Type 8
50:
RNA Viruses
PICORNAVIRUSES A
Enteroviruses
Newer Enteroviruses (68 to 72)
Rhinoviruses
ORTHOMYXOVIRUSES
INFLUENZA VIRUSES
PARAMYXOVIRUSES
Mumps
Respiratory Syncytial Virus
Parainfluenza Viruses
Measles
RUBELLA VIRUS
RHABDOVIRUS
Rabies Virus
ARBOVIRUS
Tick Borne Group
Bunyaviridae
Morphology
Classification
Pathogenesis
Clinical Picture
Laboratory Diagnosis
51:
Severe Acute Respiratory Syndrome (SARS)
CLINICAL PICTURE
LABORATORY DIAGNOSIS
MANAGEMENT
52:
Avian Influenza (Bird Flu)
SPREAD OF INFECTION
SYMPTOMS
PREVENTION AND TREATMENT
53:
Acquired Immune Deficiency Syndrome (AIDS)
IMPORTANT EVENTS IN AIDS
History
Structure and Properties of HIV
Laboratory Diagnosis
Direct Methods
Indirect Methods
Other Investigations
STRATEGIES OF HIV TESTING IN INDIA
Strategy I: (Blood Donation)
Strategy II: (Surveillance and Diagnosis)
Strategy III: (Diagnosis)
54:
Miscellaneous Viruses
NONBACTERIAL GASTROENTERITIS (VIRAL GASTROENTERITIS)
ACUTE ASEPTIC MENINGITIS (VIRAL MENINGITIS)
PERINATALLY ACQUIRED VIRAL INFECTION
INTRAUTERINE INFECTION
POSTNATAL VIRAL INFECTION
PART VI: MYCOLOGY
55:
Mycology
MORPHOLOGICAL CLASSIFICATION OF FUNGI
SYSTEMATIC CLASSIFICATION
LABORATORY DIAGNOSIS
Direct Microscopy
Potassium Hydroxide (KOH) Preparation
Gram Stain
India Ink Preparation
Culture
Tissue Sections
Serology
Superficial Mycosis
Pityriasis Versicolor
Tinea Nigra
Black Piedra
Etiology: Piedra hortai
White Piedra
MICROSPORUM
Trichophyton
Trichophyton Rubrum
Diseases Produced
Trichophyton Mentagrophytes
Diseases Produced
Trichophyton Tonsurans
Trichophyton Schoenleinii
EPIDERMOPHYTON
SUBCUTANEOUS MYCOSIS
Rhinosporodiasis
Chromoblastomycosis
Mycetoma
Etiology
Sporotrichosis
SYSTEMATIC MYCOSIS
Cryptococcosis
Other Laboratory Tests
Candidiasis
Histoplasmosis
Coccidioidomycosis
Paracoccidioidomycosis
Blastomycosis
Pneumocystis Carinii
OPPORTUNISTIC MYCOTIC INFECTIONS
Aspergillus
Penicillosis
PENICILLOSIS MARNEFFEI
Zygomycosis
MISCELLANEOUS MYCOLOGY TOPICS
PART VII: PARASITOLOGY
56:
Introduction
TAXONOMY AND CLASSIFICATION
HISTORY IN BRIEF
History of Protozoa
History of Helminths
PARASITE
HOST
Association of Parasite and Host
MECHANISMS OF DISEASE PRODUCTION BY PARASITES
The Effect of Parasites on their Host
Mechanical Injury
Deleterious Action of Toxic Substances
Deprivation of Nutrients, Fluids and Metabolites
Introduction of Pathogenic Microorganisms
REACTION OF THE HOST TO PARASITE
57:
Protozoa
ENTAMOEBA HISTOLYTICA
Geographical Distribution
Habitats
Morphology
Trophozoite
Precystic Stage
Pathogenesis
Pathology
Characters of Amebic Intestinal Ulcers
Laboratory Diagnosis
SEROLOGICAL TESTS IN INVASIVE AMEBIASIS
Treatment
Non-pathogenic Amebae
Entamoeba gingivalis
Endolimax nana
Iodameba butschlii
FREE LIVING SOIL AMEBA
Primary Amebic Meningoencephalitis (PAM)
Etiologic Agent
GRANULOMATOUS AMEBIC ENCEPHALITIS
Etiologic Agent
Clinical Picture
Laboratory Diagnosis
Treatment
GIARDIA LAMBLIA
Geographical Distribution
Habitat
Morphology
Symptomatology
Pathology
Laboratory Diagnosis
Treatment
Prevention
TRICHOMONAS VAGINALIS
Geographical Distribution
Habitat
Morphology
Mode of Transmission
Incubation Time
Pathogenesis and Pathology
Clinical Picture
Laboratory Diagnosis
Treatment
TRICHOMONAS TENAX
TRICHOMONAS HOMINIS
TRYPANOSOMA BRUCEI
Geographical Distribution
Habitat
Morphology
Life Cycle
Clinical Picture
Laboratory Diagnosis
Treatment
TRYPANOSOMA CRUZI
Geographical Distribution
Habitat
Morphology
Clinical Picture
Laboratory Diagnosis
LEISHMANIA DONOVANI
Geographical Distribution
Habitat
Morphology
Amastigote Form
Promastigote Form
Culture
Animal Models
Mode of Transmission
Life Cycle
Pathology and Clinical Picture
Spleen
Liver
AIDS and Visceral Leishmaniasis
Laboratory Diagnosis
Direct Agglutination Test (DAT)
Treatment
Preventive Measures
POST KALA-AZAR DERMAL LEISHMANIASIS (DERMAL LEISHMANIASIS)
Geographical Distribution
Pathogenesis and Clinical Picture
Laboratory Diagnosis
Treatment
LEISHMANIA TROPICA
Geographical Distribution
Habitat
Morphology
Clinical Features
Pathology
Laboratory Diagnosis
LEISHMANIA BRASILIENSIS
Geographical Distribution
Habitat
Morphology
Life Cycle
Clinical Features
Pathology
Laboratory Diagnosis
MALARIAL PARASITE
GEOGRAPHICAL DISTRIBUTION
Habitat
Life cycle in Man (Schizogony)
Pathogenesis of Plasmodium
Clinical Picture
Complications of P. falciparum
Transmission Modes
Immunity
Liver
Macroscopic (Gross)
Microscopic
Spleen
Macroscopic
Microscopic
Kidney
Macroscopic
Microscopic
Bone Marrow
Macroscopic
Microscopic
Brain
Macroscopic
Microscopic
Heart
Lungs
Gastrointestinal Tract
Laboratory Diagnosis
Treatment
Prevention and Control
TOXOPLASMA GONDII
Geographical Distribution
Habitat
Morphology
Tissue Cysts
Oocysts
Life Cycle
Enteric Cycle in Cat
Exoenteric Cycle in Man
Mode of Transmission
Pathogenesis
Clinical Picture
Laboratory Diagnosis
Treatment
ISOSPORA BELLI
Morphology
Life Cycle (Fig. 57.10)
Pathogenicity
Laboratory Diagnosis
Treatment
CRYPTOSPORIDIUM PARVUM
Geographical Distribution
Morphology
Life Cycle
Pathogenesis and Clinical Picture
Laboratory Diagnosis
Treatment
BABESIA
Geographical Distribution
Habitat
Morphology
Transmission of Infection
Incubation Period
Pathogenesis and Clinical Features
Laboratory Diagnosis
Treatment
PARASITES ASSOCIATED WITH AIDS
BALANTIDIUM COLI
Geographical Distribution
Habitat
Morphology
Cyst (Fig. 57.11)
Pathology
Laboratory Diagnosis
Prophylaxis
58:
Helminths
TRICHINELLA SPIRALIS
Geographical Distribution
Habitat
Morphology
Pathogenicity and Clinical Picture
Laboratory Diagnosis
Treatment
TRICHURIS TRICHIURA (WHIPWORM)
Geographical Distribution
Habitat
Morphology
Pathogenicity and Clinical Picture
Laboratory Diagnosis
Treatment
ASCARIS LUMBRICOIDES (ROUNDWORM)
Geographical Distribution
Habitat
Morphology
Adult Worm
Egg Fertilized
Unfertilized
Life Cycle
Pathogenicity and Clinical Picture
Laboratory Diagnosis
Treatment
ANCYLOSTOMA DUODENALE (HOOKWORM)
Geographical Distribution
Habitat
Morphology
Adult Worm
Egg
Life Cycle
Pathogenicity and Clinical Picture
Laboratory Diagnosis
Indirect Evidences
Treatment
NECATOR AMERICANUS (TABLE 58.1)
Geographical Distribution
STRONGYLOIDES STERCORALIS
Geographical Distribution
Habitat
Morphology
Life Cycle
Pathogenesis and Clinical Picture
Laboratory Diagnosis
Treatment
ENTEROBIUS VERMICULARIS (THREADWORM)
Geographical Distribution
Habitat
Morphology
Life Cycle
Pathogenesis and Clinical Picture
Laboratory Diagnosis
Treatment
DRACUNCULUS MEDINENSIS (GUINEA WORM)
Geographical Distribution
Habitat
Morphology
Life Cycle
Pathogenesis and Clinical Picture
Laboratory Diagnosis
Treatment
WUCHERERIA BANCROFTI
Geographical Distribution
Habitat
Morphology
Adult Worm
Microfilaria
Life Cycle
Clinical Picture and Pathogenesis
Laboratory Diagnosis
Treatment
BRUGIA MALAYI
Geographical Distribution
Habitat
Morphology
Life Cycle
Pathogenicity and Clinical Picture
Treatment
ONCHOCERCA VOLVULUS
Geographical Distribution
Habitat
Morphology
Life Cycle
Pathogenicity and Clinical Picture
Laboratory Diagnosis
Treatment
LOA LOA
Geographical Distribution
Habitat
Morphology
Life Cycle
Pathogenicity and Clinical Picture
Treatment
MANSONELLA OZZARDI
Geographical Distribution
Habitat
Morphology
Pathogenicity
Laboratory Diagnosis
DIPHYLLOBOTHRIUM LATUM (FISH TAPEWORM)
Geographical Distribution
Habitat
Morphology
Life Cycle (Fig. 58.1)
Clinical Picture
Laboratory Diagnosis
Treatment
Prophylaxis Prevention
TAENIA SAGINATA (BEEF TAPEWORM)
Geographical Distribution
Habitat
Morphology (Fig. 58.2)
Adult Worm
Larvae
Egg
Life Cycle
Clinical Picture
Laboratory Diagnosis
Treatment
TAENIA SOLIUM (PORK TAPEWORM)
Geographical Distribution
Habitat
Morphology (Table 58.3)
Adult worm (Fig. 58.2)
Larva (Fig. 58.3)
Eggs (Fig. 58.3)
Life Cycle
Clinical Picture
Diagnosis
Treatment
CYSTICERCUS CELLULOSAE
Morphology
Transmission
Clinical Features
Laboratory Diagnosis
Treatment
ECHINOCOCCUS GRANULOSUS (DOG TAPEWORM)
Geographical Distribution
Habitat
Morphology
Adult Worm
Eggs
Larval Form
Life Cycle
Hydatid Cyst (Fig. 58.4)
Ectocyst
Endocyst
Hydatid Fluid
Hydatid Sand
Formation of Daughter Cysts
Animal Model
Immunology
Clinical Features
Laboratory Diagnosis
Treatment
ECHINOCOCCUS MULTILOCULARES
HYMENOLEPIS NANA (DWARF TAPEWORM)
Geographical Distribution
Habitat
Morphology
Eggs
Life Cycle (Fig. 58.5)
Clinical Picture
Laboratory Diagnosis
Treatment
SCHISTOSOMA
General Clinical Symptoms
SCHISTOSOMA HEMATOBIUM (BILHARZIA HEMATOBIUM)
Geographical Distribution
Habitat
Morphology
Life Cycle (Fig. 58.7)
Pathogenicity and Clinical Features
Laboratory Diagnosis
Treatment
59:
Medical Entomology
PART VIII: CLINICAL MICROBIOLOGY
60:
The Normal Flora
61:
Collection and Transport of Clinical Specimens
62:
Collection and Preliminary Processing of Specimens
63:
Diagnostic Microbiology—An Approach to Laboratory Diagnosis
PYREXIA OF UNKNOWN ORIGIN (PUO)
SORE THROAT
Etiology
Collection of Specimen
Culture
URINARY TRACT INFECTION
Causes
Collection of Specimens
Cultural Procedure
MENINGITIS
Causes
Collection of Specimen
Processing and Culture of Specimen
INFECTIVE DIARRHEA
Causes
Further Processing and Culture Procedure
WOUND INFECTION
Etiology
FOOD POISONING
Etiology
PULMONARY TUBERCULOSIS
Sputum
Laryngeal Swab
Gastric Lavage
Infective Endocarditis
Causes
Collection of Specimen
Culture Procedure
SEPTICEMIA/BACTEREMIA
Laboratory diagnosis
Etiology of septicemia
64:
Rapid and Automation Methods in Diagnostic Microbiology
65:
Molecular Techniques in Microbiology
INTRODUCTION
AMPLIFIED TESTS
NONAMPLIFIED TESTS
Nucleic Acid Probe
Molecular Techniques in Clinical Laboratory
66:
Serological and Skin Tests
67:
Microbiology in the Service of Human Being
MEDICAL MICROBIOLOGY
ROLE OF MICROBIOLOGY
IMPORTANCE IN DIAGNOSIS OF INFECTIOUS DISEASE
68:
Community Microbiology
69:
Emerging and Re-emerging Microbial diseases
EMERGING MICROBIAL DISEASES
PANDEMIC OF SWINE FLUE (H1N1) OUTBREAK OF APRIL 2009
RE-EMERGING MICROBIAL DISEASES
FACTORS RESPONSIBLE FOR EMERGING AND RE-EMERGING OF MICROBIAL DISEASES
Control and Prevention
70:
Nosocomial Infections
PATIENTS REQUIRING ISOLATION
HOSPITAL INFECTION AND PREVENTION
EPIDEMIOLOGICAL MARKERS USEFUL IN INVESTIGATING HOSPITAL INFECTION
PREVENTION OF NOSOCOMIAL INFECTIONS
FACTORS RESPONSIBLE FOR HOSPITAL INFECTIONS
SOURCES OF HOSPITAL INFECTION
MODE OF INFECTION
OUTLINE OF INVESTIGATIVE APPROACH OF HOSPITAL INFECTION OUTBREAK
71:
Hospital and Laboratory Waste
INFECTIOUS WASTE OF HOSPITAL AND LABORATORY
INFECTIOUS MICROORGANISMS ASSOCIATED WITH HOSPITAL OR LABORATORY
SEGREGATION OF HOSPITAL WASTE
TECHNIQUES FOR WASTE TREATMENT
Chemical Method to Dispose of Hospital Waste
Incinerator
Advantages
Disadvantages
Autoclave
Uses
Advantages
Disadvantage
Microwaving
Uses
Advantage
Disadvantages
Hydroclaving
Advantages
Plasma Technology
Advantages
DISPOSAL OF HOSPITAL AND LABORATORY WASTE
72:
Diagnostic Virology
73:
Emergency Microbiology
VISUALIZATION OF THE AGENT OF DISEASE
ANTIGEN DETECTION
Detection of Substances Produced by the Infecting Organisms in Specimens
Detection of Substances Produced by the Host in Response to Infection
74:
Bacteriology of Milk, Air and Water
BACTERIOLOGY OF MILK
Sources of Bacteria in Milk
Milk-borne Diseases
Pasteurization of Milk
Biological Standards of Milk
Bacteriologic Examination
BACTERIOLOGY OF AIR
Indications of Bacteriological Examination of Air
Methods of Bacteriological Examination of Air
Settle Plate Method
Slit Sampler Method
Bacteriological Examination of Environment Dust
Air Centrifugation
Sweep Plate Method
Dust Sampling
Interpretation
BACTERIOLOGY OF WATER
Bacterial Flora of Water
Natural Flora of Water
Mixed with Soil
Mixed with Sewage
Sewage Proper Bacteria
Water-borne Diseases
Bacterial
Viral
Protozoal
Helminths
Aquatic Host
Indicators Bacteria of Human Pollution (Intestinal/Feces) of Water
Coliforms
Sampling of Water
Types of Water to be Sampled
Water Sample from Tap
Sample from Reservoir
Sample from Well
Presumptive Coliform Test (Fig. 74.1 and Table 74.1)
Detection of Fecal Streptococci and Clostridium perfringens
Colony Count
Viruses in Water
PART IX: APPENDICES
Appendices
INDEX
TOC
Index
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