Manual of Practical Medical Biochemistry Evangeline Jones
INDEX
A
Abnormal
chemical constituents of urine 111
constituents 93
Absorption photometry 121
Actual volume of blood 127, 132, 137, 146
Advantages of Benedict’s test over Fehling’s test 4
Albumin 48
Albuminuria 112
Aldehyde test 46
Aliphatic amino acids 41
Alkaline
copper reduction methods 124
hypobromite test 110
Amino acid 41
Ammonia 107
Ammonium
molybdate 167
sulphate 165
Analysis of
abnormal constituents of urine 114
bile 95
cerebrospinal fluid 160
milk 90
normal urine 109
Aromatic amino acids 41
Autoanalyser methods 124, 131
b
Barfoed’s test 4, 7
Beer’s law 121
Benedict’s
qualitative reagent 167
reagent 3
test 3, 4, 7, 112, 114
uric acid
reagent 168
test 75, 111
Benzene ring 41
Benzidine
powder 165
test 113, 115
for blood 93
Bial’s
reagent 3
test 3, 7
Bile 94
pigments 114
salts 113
Biuret test 45
Blood 113, 120
c
Calcium 106
Carbohydrates 40
Carboxy hemoglobin 165
Centrifuge 163, 172
Cerebrospinal fluid 159184
Charts 175
Chemicals 165
Chloride 106
Choice of blood specimen 125
Chromatography paper 170
Classification of jaundice 156
Cole’s mercuric nitrite test 46
Collection of blood 120
Color reactions of
casein 91
proteins 45
Colorimeter 162, 174
Colorimetry 121
Congenital hyperbilirubinemia 157
Conjugated hyperbilirubinemia 157
Copper sulphate 165
Creatinine 76, 108
urine 157
picrate 136
d
Decreased level of uric acid 149
Determination of alkaline phosphatase 158
Development of color 136
Diabetes mellitus 182
Diacetyl monoxime method 131, 134, 166
Diamino-dicarboxylic amino acid 41
Diamino-mono-carboxylic amino acids 41
Direct colorimetric method 131
Dolichos uniflorus 166
e
Electrophoresis 163
Electrophoretogram 169
Emulsoids 42
End-point analysis 123
Enzymatic methods 124
Enzyme levels 184
EPP-cirrhosis of liver 169
EPP-multiple myeloma 170
EPP-nephrotic syndrome 169
Estimation of
blood sugar 124, 125
creatinine 138
direct and total bilirubin 156
fluid protein 159
phosphorus 150
serum
alkaline phosphatase 158
chloride 151
phosphorus 149
plasma proteins 142
total proteins 142
uric acid 144
SGOT 154
sodium and potassium in serum 173
total cholesterol in serum 152
urea 131, 134
uric acid 146
in serum 144
Ethereal sulphates 107
f
Factors affecting bile secretion 95
Fehling’s
reagent 3
test 3, 4, 7
Ferricyanide reduction methods 124
Filters 122
Flame photometer 172
Flowers of sulphur 167
Folin-Wu
method 126
tube 125
Formation of osazone 5
Fouchet’s
reagent 168
test 96, 114, 115
for bile pigments 93
Foulger’s
reagent 2, 168
test 2, 7
Fructosan 2
Fructosazone 164
Full saturation test 6
Functional proteinuria 159
Functions of bile 95
g
Gastric juice 92
Gerhardt’s test 113
Gestational diabetes mellitus 182
Glucometer 125
Glucosazone 164
Glucose 183
oxidase 124
peroxidase method 128
tolerance test 181
Gmelin’s test 96, 114
Gout 149
h
Haemin crystals 164
Half saturation and full saturation test 6
Hay’s test 113, 115
Heat coagulation test 112, 115
Heller’s test 113, 115
Hemoglobin 98
Heterocyclic amino acids 41
Hormones 184
Horse gram powder 166
Hydrochloric acid 93
Hyperglycemia 129
Hypoglycemia 129
Hypouricemia 149
i
Imino acid 41
Impaired glucose tolerance 181
Indicator paper 162
Inorganic constituents 107, 108
Instruments 172
Interpret chart 175
Iodine test 6, 7
for starch 93
j
Jaffe’s
reaction 157
test 76, 111
k
Ketone bodies 113
Kinetic analysis 123
l
Lactometer 162
Lactosazone 164
Lactose 1
Lambert’s law 121
Lead acetate 166
Liebermann-Burchard reaction 86, 87
Lipid profile 183
Lipids 86
m
Maclean’s test 94
Maltosazone 164
Maltose 1
Manual methods 124
Methemoglobin 99, 164
Methods of estimation 131
Methods of estimation of
cholesterol 152
creatinine 140
plasma proteins 142
serum chlorides 151
uric acid 144
Methods of fractionation of plasma proteins 142
Milk 90
Mixed hyperbilirubinemia 157
Modified Folin-Wu method 125
Molisch’s test 2, 7, 47
Mono-amino-dicarboxylic amino acids 41
Mono-amino-mono-carboxylic amino acids 41
Monosaccharides 1
Murexide test 76
n
Neumann’s test for phosphoprotein 91
Neutral salt 165
Ninhydrin test 46
Nippe’s fluid 168
Non-protein nitrogenous substances 75
Normal
composition of CSF 159
constituents 93
daily urea excretion in urine 135
level of glucose 128
urine 108
values 183
o
Oligosaccharides 1
Operation of pH meter 174
Optical density 122
Organic
constituents 107, 108, 110
proteinuria 160
Osazone test 4, 6, 7
O-toludine method 124
Oxyhemoglobin 99, 164
p
Paper
chromatography 177
electrophoresis 178
Parts of
apparatus 173
colorimeter 122
pH meter 174
Pauly’s test 47
Peptides 41
Peptones 42
Pettenkofer’s test 96, 113
pH meter 162, 174
pH paper 162
Phenol red 167
Phenolphthalein 167
Phenyl hydrazine hydrochloride 165
Phosphates 106
Phosphomolybdic acid 166
Photo electric colorimeter 162
Picric acid 165
Polysaccharides 1
Potassium oxalate 167
Precipitation by
alkaloidal solution 44
concentrated salt solution 44
heat and acid 45
heavy metals 43
Precipitation of milk 90
Precipitation reactions of proteins 42
Preparation of
hemin crystals 104
protein free filtrate 126, 131, 136
Preservatives for urine 121
Primary proteoses 42
Protein analysis in CSF 160
Proteins 41, 42
q
Qualitative analysis
carbohydrates 1
gastric juice 93
Qualitative tests for
carbohydrates 2
lipids 86
proteins 42
Quantitative
analysis 120
experiments 124
r
Reactions for cholesterol 86
Reactions of
creatinine 76
disaccharides 5
lipids 87
milk 90
monosaccharides 7, 11
polysaccharides 6
proteins albumin 48
urea 75
uric acid 75
Reagents required 127
Reduced hemoglobin 99
Reduction tests 5
Rothera’s test 113, 114
Ryle’s
stomach tube 163
tube 163
s
Sakaguchi test 46
Salkowski reaction 86, 87
Schiff’s test 76, 111
Secondary proteoses 42
Seliwanoff’s
reagent 168
test 2, 7
Separation of
amino acids 177
serum protein 178
Serum 184
glutamate oxaloacetate transaminase 154
Silver nitrate 166
Sodium
fluoride 167
hypobromite
solution 168
test 75
Solid phase tests for glucose 124
Specific
color reactions 46
gravity measurement 90
urease test 75, 110
Specimen collection 104
Spectroscope 163
Spotters 162
Stick tests 131
Structure of
cholesterol 170
fructose 171
glucose 171
Sucrose 1
hydrolysis test 5, 7
Sulphates 107
Sulphosalicylic acid 115, 166
test 112
Sulphur
powder 167
test 47
Suspensoids 42
Syllabus for charts 175
t
Test for
ammonia 110
bile 93
bile
pigments 114, 115
salts 113, 115
blood 115
in urine 113
calcium and phosphorus 92, 109
casein 91
chloride 109
creatinine 111
fat 91
inorganic sulphates 110
ketone bodies 113, 114
lactic acid 94
lactose 91
proteins 96, 115
reducing sugar 112
unsaturation 86
urea 110
uric acid 111
urobilinogen 111, 114
Test with precipitate 91
Tests based on reducing property 3
Tests for
bile pigments 96
fructose 11
glucose 7
proteins 112
Titration method using iodimetry 124
Tollen’s Orcinol test 3
Topfer’s
reagent 168
test for hydrochloric acid 93
Types of
centrifuge 172
proteinuria 112
u
Ufflemann’s test 94
Unconjugated hyperbilirubinemia 156
Urea 75, 107
Urease methods 131
Uric acid 75, 108
Urine 104, 121, 159, 184
Urinometer 162
Urobilinogen 108, 114
v
Various precipitation methods of proteins 43
x
Xanthoproteic test 46
×
Chapter Notes

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