Manual of Intrauterine Insemination (IUI) In Vitro Fertilization (IVF) and Intracytoplasmic Sperm Injection (ICSI) Kanthi Bansal
INDEX
ABCDEFGHIKLMNOPQRSTUVWZ
A
Acrosomal integrity 37
Advantages of vitrification 57
Aims of IUI 3
Alpha cannula positioned for intrauterine insemination 14
Androstenedione 31
Antagonist protocol 76
Antisperm antibodies 5, 66
Artificial insemination 16
Assessment of maturity of oocytes 87
Assisted reproductive techniques 86
Asthenozoospermia 4
Azoospermia 16
B
Basic requirements for IUI 8
Bicornuate uterus 65
Bilateral tubal block 110
Bleeding 80
Blocked single fallopian tube 5
Bromocryptine 6
C
Calculation of total motile sperm 41
Calf pain 48
Carbon dioxide cylinder 10
Catheters 14
used for IUI 15
Cell
number 86
regularity 86
Centrifuge 10
Cervical
incompatibility 65
mucus hostility 5
Cervix 5
Chest pain 48
Chlamydia trachomatis 17
Classification of OHSS 46
Clinical aspects of cryopreserved embryos 55
Clomiphene citrate 6, 7, 73
Congenital bilateral
absence of vas deferens 93
vas aplasia 94
Corticosteroids 6
Cotton swabs 12
Cryocans 11
Cryogloves 52
Cryopreservation 51, 53, 109
of human sperms 51
of semen 53
Culture media 12
Cusco’s speculum 12
Cytomegalovirus 17
D
Dark urine 48
Degree of fragmentation 87
Density gradient
method 43
technique 43
Development of sperms 28
Diarrhea 48
Dimethyl sulfoxide 51, 54
Direct intraperitoneal insemination 1
Disposable plastic gloves 12
Donation of eggs and embryos 63, 64
Donor insemination 18, 16
Dopamine agonists in management of OHSS 50
Drugs to stimulate ovaries 72
Dry
hair 48
skin 48
Duration of infertility 6
E
Earle’s medium 43
Economics of IVF and ICSI 107
Ectopic pregnancy 106
Ejaculatory failure 4
Embryo
freezing 53
grading 88
in 4 cell stage 89
transfer 1, 16, 46, 68, 69, 100
catheters 101
Endocrinal regulation of menstrual cycle 27
Endometriosis 5, 63, 64, 110
Epididymal sperm retrieval 70, 94
Estradiol 27
Estrogen 28, 31
Ethanediol 51
Etiology of infertility 6
F
Failed donor insemination 63, 64
Fallopian tubes 5
Fertile period 13
Fertility counselors 58
Fertilization 68, 69, 82
rates for ICSI 84
Ficoll 51
Follicle stimulating hormone 27, 61
Follicular phase 26
Formation of oocyte 24
Freezing eggs 57
French protocol 76
Fructose level test 36
FSH and LH 28
Fyrete device 10
G
Gastrointestinal tract 46
Glycerol 51
Gonadotropin
releasing hormone 6, 28
H
Handling of specimen 34
Heating block 11
Herpes simplex virus 17
Highly purified FSH/recombinant FSH 73
Hormonal monitoring 77
Human
chorionic gonadotropin 6
menopausal gonadotropins 61, 73
Hypo-osmotic swelling test 37
Hypospadias 4
I
Iatrogenic obstruction-hydrocele repair 94
Immotile sperm 66
In vitro fertilization 1, 16, 46, 68
Indications
and patient selection in ICSI 66, 91
for surgical sperm retrieval for ICSI 70
Insemination device 12
Interstitial cell stimulating hormone 32
Intracytoplasmic sperm injection 16, 61, 68, 84, 90, 104
Intrauterine
adhesions 65
insemination 1, 19, 61
room 8
with donor semen 5
IVF and ICSI 61
K
Kidney tray 12
L
Laboratory
aspects 103
discipline 9
set-up and equipment 8
Laparoscopic oocyte retrieval 78
Liquid nitrogen 12
Loading of prepared sperms 15
Lower abdominal pain 48
Luteal phase defect 65
Luteinizing hormone 27, 32
M
Maintenance of lab sterility 104
Making IVF affordable 108
Makler’s chamber 11
Male
factor 4, 63
infertility 114
orchidectomy 52
Mammalian fertilization 38
Medical termination of pregnancy 63
Medically assisted reproductive techniques 2, 51
Meiosis in oocyte 24
Menstrual cycle 26
Metformin 6
Methods of
cryopreservation 51
sperm preparation 17
Microsurgical
epididymal sperm aspiration 94, 95
selective biopsy technique 97
Mild
endometriosis diagnosed by laparoscopy 22
hyperstimulation 46
Moderate hyperstimulation 46
N
Native prewash semen analysis 20
Natural cycle IUI 6
Nausea 48
Necrospermia 66
Non-obstructive azoospermia 70, 94
Nonsurgical methods of sperm retrieval 70, 97
Nontooth dissecting forceps 12
O
Obstructive azoospermia 70, 94
OHSS management 48
Oligoasthenoteratozoospermia 4
Oligoasthenozoospermia 4
Oligozoospermia 4
Oocyte 23
cumulus complex 26, 84, 85
grading 86
identification and grading 82
retrieval 68, 78
surrounded by sperms 83
Oogenesis 23
Open
fine needle aspiration 94, 95
method for retrieving epididymal sperm 70, 94
Organic anejaculation 70, 97
Orgasmic anejaculation 70, 97
Ovarian
hyperstimulation syndrome 6, 46, 50, 62, 106
stimulation and IUI 7
Ovulatory disorders 63, 64
P
Paper napkins 12
Partial zone dissection 91
PCOS 5, 20
Pelvic inflammatory disease 63
Percutaneous epididymal sperm aspiration 94
Performing
MESA 95
needle aspiration biopsy 96
OFNA 95
PESA 96
TEFNA 96
testicular sperm aspiration 96
Phase contrast light microscope 10, 11
Pipette pump 11
Polycystic ovarian syndrome 47
Polyethylene glycol 51
Powder free latex rubber gloves 12
Prediction of OHSS 47
Preparation
for semen examination 35
from fresh frozen sample 17
with frozen prepared semen sample 17
Primary oocyte 23
Procedure
for embryo thawing 56
for freezing of embryos 54
of embryo
loading 100
transfer 101
Procuring semen sample 33
Progesterone 27, 28
Programmed cryopreservation 54
Pronuclear oocyte 24
Propanediol 51
Psychological anejaculation 97
Pure follicle stimulating hormone 73
Q
Qualities of counselor 59
Qualities of egg 5
R
Radiotherapy/chemotherapy 52
Rate of spontaneous abortion 106
Rationale of IUI 13
Record maintenance room 8
Requirements for insemination 12
Retrieving epididymal sperm 94
Retrograde ejaculation 4, 70, 98
Role of cervix in natural conception 3
S
Safal Fertility Foundation 42, 108
Screening of donors 16
Secondary oocyte 23
Semen
analysis 19, 33
collection 40
containers 12
jar 33
parameters 88
wash 40
Severe
hyperstimulation 46
male factor infertility 62
oligoosthenospermia 66
Severe structural abnormalities of sperm 66
Sex-linked diseases 63, 65
Sim’s speculum 12
Single seminiferous tubule technique 97
Situational anejaculation 70, 97
Slide warmer 10
Sonographic monitoring 76
Sperm 31
count 35
cryopreservation 16
function test 36, 37
morphology 36
preparation techniques 40
retrieval techniques 69, 94
Spermatogenesis and sperms 28
Stages of spermatogenesis 30
Steps
in ICSI 69
of IVF and ICSI 68
of thawing 56
Stereozoom microscope 82
Stimulation 72
in donor insemination 17
of ovaries 68
protocols 74, 75
in IUI 6
Storing donor semen 51
Subzonal insemination 91
Superovulation 72
Surgical methods of sperm retrieval 70, 94
Swim-up technique 41, 42
T
Tamoxifen 6
Technique of IUI 13
Teratozoospermia 4
Test tube
stand 11
warmer 11
Testicular
hormones 31
sperm retrieval 96, 97
Testosterone 31
Thawing of embryos 56
Thin endometrial lining 65
Timing of hCG administration 77
Tissue paper 12
Total
astheno/necrozoospermia 70, 94
motile sperm 41
quality management 89
sperm
count 35
number 35
Treatment of OHSS 50
U
Ultrashort protocol 76
Ultrasonography 49
Ultrasound
guided oocyte retrieval 79
machine 8
Unexplained infertility 4, 19, 21, 63, 64, 113
Uterine
fibroids 65
polyps 65
problems 63, 65
V
Vacuum suction machine 79
Vaginismus 5
Vas deferens 93
Vascular endothelial growth factor 47
Vessel for cryopreservation 52
Viscous semen 4
Vitrification 56
Vomiting 48
W
Wash bottles 12
Wheel pipette 11
Z
Zona-free Hamster egg penetration test 38
Zygote 24
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Chapter Notes

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