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Practical Biochemistry for BDS Purvi Purohit

SECTION 1: INTRODUCTION TO PRACTICAL BIOCHEMISTRY
CHAPTER 1:Biochemistry in Dentistry
CHAPTER 2:A Peep into the Biochemistry Laboratory
- COMMON LAB EQUIPMENT AND INSTRUMENTS
CHAPTER 3:Quality Control and Precision of Results
- IMPORTANCE OF QUALITY CONTROL IN CLINICAL PRACTICE
CHAPTER 4:How to Work in a Biochemistry Laboratory
- INSTRUCTIONS FOR WORKING IN A BIOCHEMISTRY LABORATORY
SECTION 2: QUALITATIVE EXERCISES
CHAPTER 5:Carbohydrate Analysis
- DEFINITION
  - Classification
- TESTS FOR ANALYSIS OF CARBOHYDRATES
- TESTS FOR POLYSACCHARIDES
- IDENTIFICATION OF UNKNOWN CARBOHYDRATE
- VIVA VOCE
CHAPTER 6:Lipid Analysis
- DEFINITION
  - Classification
- ANALYSIS OF LIPIDS BASED ON PHYSICAL PROPERTIES
- ANALYSIS OF DERIVED LIPIDS
- VIVA VOCE
CHAPTER 7:Protein Analysis
- DEFINITION
  - Classification
- GROUP SPECIFIC TEST OF PROTEINS
- COLOR REACTIONS FOR IDENTIFICATION OF VARIOUS AMINO ACIDS
- PRECIPITATION OF PROTEINS
- IDENTIFICATION OF UNKNOWN PROTEIN
- VIVA VOCE
SECTION 3: CLINICAL BIOCHEMISTRY
CHAPTER 8:Gastric Juice Analysis
- ANALYSIS OF GASTRIC JUICE
  - Method of Collection of Gastric Juice
- DETERMINATION OF FREE AND TOTAL ACIDITY
- OBSERVATION TABLE
- VIVA VOCE
CHAPTER 9:Colorimetry
- PHOTOMETRY
- VIVA VOCE
CHAPTER 10:Blood Sugar
- ESTIMATION OF BLOOD GLUCOSE BY ORTHOTOLUIDINE METHOD OF DUBOWSKI, (1962)
- ESTIMATION OF BLOOD GLUCOSE BY GLUCOSE OXIDASE OR PEROXIDASE
- VIVA VOCE
CHAPTER 11:Total Protein and A:G Ratio
- ESTIMATION OF TOTAL PROTEIN BY BIURET METHOD
- ESTIMATION OF ALBUMIN BY BCG METHOD
- VIVA VOCE
CHAPTER 12:Serum Urea
- ESTIMATION OF SERUM UREA BY BERTHELOT METHOD
- VIVA VOCE
CHAPTER 13:Serum Creatinine
- ESTIMATION OF SERUM CREATININE (BY JAFFE’S METHOD)
- VIVA VOCE
CHAPTER 14:Serum Lipid Profile
- ESTIMATION OF SERUM CHOLESTEROL (ENZYMATIC METHOD)
- ESTIMATION OF HDLC
  - Method: Mg–Phosphotungstic Acid Precipitation (Gordon T et al 1977)
- COMPONENTS OF PRECIPITATING REAGENTS
- ESTIMATION OF TRIGLYCERIDES
- VERY LOW DENSITY LIPOPROTEIN CHOLESTEROL
  - Normal Values
- LOW DENSITY LIPOPROTEIN CHOLESTEROL
- VIVA VOCE
CHAPTER 15:Estimation of Sugar in Urine (Benedict’s Quantitative Method)
- PRINCIPLE
- VIVA VOCE
SECTION 4: URINE ANALYSIS
CHAPTER 16:Analysis of Normal Urine
- TEST FOR ORGANIC CONSTITUENTS
- TEST FOR INORGANIC CONSTITUENTS
- VIVA VOCE
CHAPTER 17:Analysis of Abnormal/Pathological Urine
- PROTEINS
- DETECTION OF PROTEINS IN URINE
- DETECTION OF SUGAR IN URINE
- DETECTION OF KETONE BODIES
  - Rothera’s Test
- DETECTION OF BILE PIGMENTS AND BILE SALTS
- DETECTION OF BLOOD
- VIVA VOCE
SECTION 5: APPLICATIONS OF CLINICAL BIOCHEMISTRY
CHAPTER 18:Liver Function Tests
- TESTS BASED ON BILIRUBIN METABOLISM
- TESTS BASED UPON METABOLIC FUNCTION OF LIVER
- TEST-BASED SYNTHESIS OF PLASMA PROTEINS
  - Clinical Interpretations
- TESTS BASED ON DETOXICATION ACTION OF LIVER
  - Clinical Interpretations
- TESTS BASED ON EXCRETORY FUNCTION OF LIVER
  - Clinical Interpretations
- TESTS BASED ON THE SYNTHESIS OF CLOTTING FACTORS BY LIVER
- ENZYME ASSAYS
  - Serum Transaminases
- ALKALINE PHOSPHATASES—3–13 KÅ UNITS/100 ML (23–92 IU/L)
- VIVA VOCE
CHAPTER 19:Renal Function Tests
- TEST FOR ASSESSMENT OF GLOMERULAR FUNCTION
- TESTS FOR MEASURING RENAL PLASMA FLOW
- TESTS FOR TUBULAR FUNCTION
- VIVA VOCE
CHAPTER 20:Thyroid Function Tests
- HORMONE CONCENTRATION
- BINDING PROTEINS
- THYROTROPIN RELEASING HORMONE RESPONSE TEST
- SERUM CHOLESTEROL
- THYROID AUTOANTIBODIES
- THYROID SCAN
- VIVA VOCE
CHAPTER 21:Parathyroid Function Tests
- PTH LEVEL
- SERUM CALCIUM LEVEL
  - Principle
- SERUM PHOSPHOROUS
  - Principle
- VITAMIN D
- BONE SCAN
- VIVA VOCE
SECTION 6: DEMONSTRATIONS
CHAPTER 22:Analysis of Saliva
- COMPOSITION OF SALIVA
- ANALYSIS OF SALIVA
- INORGANIC CONSTITUENTS ANALYSIS
- VIVA VOCE
CHAPTER 23:Analysis of Food–Stuffs
- MILK
- ANALYSIS OF MILK
  - Physical Examination of Milk
  - Chemical Examination of Milk
- VIVA VOCE
CHAPTER 24:Biochemical Techniques
- CHROMATOGRAPHY
- TYPES OF CHROMATOGRAPHY
- ELECTROPHORESIS
- VIVA VOCE
CHAPTER 25:Titratable Acidity of Urine
- DETERMINATION OF TITRATABLE ACIDITY
  - Calculation
  - Normal Values and Clinical Importance
- VIVA VOCE
CHAPTER 26:Nonprotein Nitrogen
- PRINCIPLE
- REAGENT
- VIVA VOCE
CHAPTER 27:Recent Advances in Clinical Biochemistry
- SPECTROSCOPY
- GENE IDENTIFICATION TECHNIQUES
  - Southern Blotting
  - DNA Microarrays
- GENE MULTIPLICATION TECHNIQUES
- GENE STORAGE TECHNIQUES
CHAPTER 28:Preparation of Various Reagents
- BENEDICT’S QUALITATIVE SOLUTION
- REAGENTS FOR QUANTITATIVE ANALYSIS
CHAPTER 29:Normal Reference Values of Various Biochemical Parameters

INDEX

TOC
Index

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Chapter Notes