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Manual on IUI: What, When and Why
Narendra Malhotra, Jaideep Malhotra, Nusrat Mahmud
CHAPTER 1:
Overview of Infertility
DEFINITIONS
Factors Responsible for Fertility
Principal Causes of Impaired Fertility or Infertility
Common Causes of Female Infertilty
Common Causes of Male Infertility
Diagnostic Procedure (According to ESHRE)
Treatment Approach of Infertile Patient
Success of Infertility Treatment
CHAPTER 2:
Intrauterine Insemination
HISTORY OF ARTIFICIAL INSEMINATION
HOW DOES IUI WORKS?
Prerequisites for IUI
Factors Influences the Success of Pregnancy in IUI
Practical Issues Involved in Enhancing the Pregnancy Outcome in IUI
CHAPTER 3:
Diagnosis of Male Infertility by Semen Quality
CHAPTER 4:
Equipment Design and Set-up of IUI Laboratory
CHAPTER 5:
Patient Selection and Management
CHAPTER 6:
Hormones and Fertility Drugs
ANTI-MÜLLERIAN HORMONE (AMH)
ANDROGENS
THYROID HORMONES
CLOMIPHENE CITRATE (CC)
LETROZOLE
METFORMIN
LUPRON (GNRH ANTAGONIST); ANTAGON, CETROTIDE (GNRH AGONISTS)
BROMOCRIPTINE/CABERGOLIN
PROGESTERONE
HUMAN CHORIONIC GONADOTROPIN (hCG)
LUVERIS (LH)
CHAPTER 7:
Controlled Superovulation
STIMULATION WITH ANTIESTROGEN
AROMATASE INHIBITORS
STIMULATION WITH GONADOTROPINS:
GONADOTROPIN PREPARATION
COMMONLY USED REGIMEN OF SUPEROVULATION
CHAPTER 8:
Role of Transvaginal Sonography in Monitoring of Ovulation and IUI
PREPARATION OF PATIENT FOR TVS
POSITION AND TECHNIQUE OF TVS (FIG. 8.1)
MONITORING OF OVULATION
TRANSVAGINAL MONITORING
ULTRASOUND MONITORING OF OVARIES:
POLYCYSTIC APPEARANCE ON USG
ULTRASONOGRAPHIC MONITORING OF ENDOMETRIAL DEVELOPMENT
CHAPTER 9:
Semen Analysis
VARIABILITY IN SEMEN PARAMETERS
SAMPLE COLLECTION AND DELIVERY
PHYSICAL CHARACTERISTICS OF SEMEN
VISUAL ASSESSMENT OF SPERM MOTILITY
OTHER MICROSCOPIC OBSERVATIONS
OTHER CELLULAR COMPONENTS AND DEBRIS
Round Cells
Epithelial Cells
Erythrocytes
Particulate debris
Measurement of Sperm Concentration
Various Chambers
Assessment of Sperm Morphology
Sperm Morphology Classification
Teratozoospermic Index (T2I)
Interpretation of Semen Parameters
Sperm Function Tests
Sperm Membrane Function
VIABILITY
Sperm Viability test
Hypo-osmotic Swelling (HOS) test
TESTS OF SPERM MEMBRANE BINDING, CAPACITATION AND PENETRATION
Hyper-activated Sperm Motility
Hemi-zona Assay
Acrosome Detection and Reaction
Triple Staining
Fluorescent Techniques
Acrosin Measurement
Gelatin Film Lysis Test
ANTISPERM ANTIBODIES
WHO GUIDELINE FOR SEMEN PARAMETERS
SPERM PREPARATION FOR IUI
Collection Methods
Retrograde Semen Sample
SEMEN ANALYSIS
Motility Evaluation
Sperm Count
Sperm Morphology
Care of the Meckler Chamber
Selection of Semen Processing Techniques
Why Sperm Wash is Needed ?
Sample Preparation for IUI
Sperm Processing Technique
Sperm Wash
Swimup Procedure
Albumin Columns Procedure:
Percoll Gradients:
Pure Sperm Preparation
Semen Processing kits
Two Layered Density Gradient Procedure (Fig. 9.8)
Single Layered Density Gradient Procedure
CHAPTER 10:
IUI Techniques
CHAPTER 11:
IUI Techniques for Ovarian Hyperstimulation Syndrome
DEFINITION
CLASSIFICATION:
SYMPTOMS
Moderate OHSS
Severe OHSS
When to see a doctor
Causes
RISK FACTORS
COMPLICATIONS
TESTS AND DIAGNOSIS
TREATMENTS AND DRUGS
Mild and Moderate OHSS
Severe OHSS
LIFESTYLE AND HOME REMEDIES
PREVENTION
Bibliography
INDEX
TOC
Index
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