Index

Practical Textbook of Biochemistry for Medical Students DM Vasudevan, Subir Kumar Das

PART A: QUALITATIVE EXPERIMENTS
1:Colour Reactions of Proteins
- EXPERIMENT 1. COLOUR REACTIONS OF PROTEINS BASED ON PEPTIDE BONDS AND TYPE OF AMINO ACID RESIDUES
2:Tests Based on Precipitation Reactions of Proteins
- PRECIPITATION BY SALTS
  - a. Half Saturation Test with Ammonium Sulphate
  - b. Full Saturation Test with Ammonium Sulphate
- ISOELECTRIC PRECIPITATION
  - 1% Casein Solution
- COAGULATION OF PROTEINS
  - 10% Egg-White or 1% Albumin Solution
- PRECIPITATION BY ORGANIC SOLVENTS
  - 10% Egg-White Solution
- PRECIPITATION BY HEAVY METALS
  - 10% Egg-White Solution
- PRECIPITATION BY ALKALOIDAL REAGENTS
  - 10% Egg-White Solution
- PRECIPITATION BY STRONG MINERAL ACIDS
  - 10% Egg White Solution
3:Analysis of Individual Proteins
- EGG-WHITE PROTEINS
- PROTEOSES AND PEPTONES
  - Physical Characters
  - Chemical Tests
- CASEIN
  - Physical Characters
  - Chemical Tests
- GELATIN
  - Physical Characters
  - Chemical Tests
4:Identification of an Unknown Protein in a Solution
5:Reactions of Monosaccharides
- CARBOHYDRATES
- MONOSACCHARIDES
  - Physical Characters
  - Chemical Tests
6:Analysis of Disaccharides
7:Analysis of Polysaccharides
8:Detection of Unknown Carbohydrate
9:Analysis of Urea
10:Uric Acid
11:Identification of Unknown Solution
12:Vitamins A and C
13:Analysis of Milk
14:Analysis of Bile
15:Analysis of Normal Constituents of Urine
- FRESH NORMAL URINE
  - Physical Character
- DETERMINATION OF SPECIFIC GRAVITY OF URINE
  - Chemical Tests
    - Inorganic Constituents
    - Tests for Organic Constituents
16:Abnormal Constituents of Urine (Report on Urine)
PART B: QUANTITATIVE EXPERIMENTS
17:Determination of Titrable Acidity and Ammonia in Urine
- PRINCIPLE
- REAGENTS
- PROCEDURE
18:Estimation of Chlorides in Urine
- PRINCIPLE
  - Reagents
- PROCEDURE
  - Clinical Significance
19:Estimation of Glucose in Urine
- PRINCIPLE
20:Colorimetry
- PRINCIPLE
- COLORIMETER
- USE OF PHOTO ELECTRIC COLORIMETER
21:Estimation of Blood Sugar
- FOLIN-WU METHOD
- ESTIMATION OF GLUCOSE BY O-TOLUIDINE METHOD
  - Principle
  - Reagents
- GLUCOSE–OXIDASE METHOD
22:Estimation of Blood Urea (By Diacetyl Monoxime Method)
- PRINCIPLE
- CLINICAL SIGNIFICANCE
23:Estimation of Creatinine
- BLOOD CREATININE
- JAFFE'S REACTION CAN ALSO BE USED TO MEASURE CREATININE IN SERUM/ PLASMA OR URINE
  - Enzymatic Method
  - Clinical Significance
24:Estimation of Uric Acid
25:Haemoglobin in Blood
- ESTIMATION
- SPECTROSCOPIC EXAMINATION OF HAEMOGLOBIN AND ITS DERIVATIVES
26:Estimation of Serum Proteins and Albumin/Globulin Ratio
- PRINCIPLE
27:Serum Calcium Estimation
- ESTIMATION BY PERMANGANATE TITRATION METHOD
- ESTIMATION BY CRESOLPHTHALEIN COMPLEXONE METHOD
28:Determination of Serum Inorganic Phosphate
- PRINCIPLE
29:Estimation of Total Cholesterol in Serum
- ZAK'S METHOD USING FERRIC CHLORIDE/ ACETIC ACID REAGENT
- ENZYMATIC METHOD
  - Clinical Significance
30:Estimation of Serum Bilirubin
- PRINCIPLE
31:Estimation of Amylase in Serum (Iodometric Method)
- PRINCIPLE
32:Estimation of Serum Alkaline Phosphatase
- METHOD OF KING AND KING USING PHENYL PHOSPHATE
- METHOD OF BESSEY ET AL USING P-NITROPHENYL PHOSPHATE
33:Estimation of Transaminase Activity
- PRINCIPLE
PART C: DEMONSTRATIONS
34:Determination of pH
- PRINCIPLE
  - Materials Required
  - Procedure
35:Estimation of Serum Electrolytes by Flame Photometry
- PRINCIPLE
- MATERIALS
  - Reagents
  - Apparatus: Flame Photometer
    - Procedure
36:Oral Glucose Tolerance Test
- MATERIALS REQUIRED
37:Paper Chromatography
- PRINCIPLE
  - Materials
  - Procedure
38:Paper Electrophoresis
39:Serum Protein Electrophoresis
40:Gastric Function Tests
- FRACTIONAL TEST MEAL
- PENTAGASTRIN STIMULATION TEST
  - Procedure
- AUGMENTED HISTAMINE TEST MEAL
- TUBELESS GASTRIC ANALYSIS
  - Clinical Significance
41:Liver Function Tests
- ZINC SULPHATE TURBIDITY TESTS
- BILIRUBIN (See CHAPTER 30)
- TRANSAMINASES (See CHAPTER 33)
- ALKALINE PHOSPHATES (See CHAPTER 32)
  - γ-glutamyl Transpeptidase
  - 5'-nucleotidase
- SERUM ALBUMIN (See CHAPTER 26)
42:Kidney Function Tests
- UREA CLEARANCE TEST
- URINE CONCENTRATION TEST
  - Osmolality and Specific Gravity
- ANALYSIS OF BLOOD (OR SERUM)
- URINE EXAMINATION
- ELIMINATION OF FOREIGN SUBSTANCES: PHENOL SULPHONPHTHALEIN (PSP) TEST
43:Pancreatic Function Test
- FUNCTIONS OF THE PANCREAS
- COMPOSITION OF PANCREATIC JUICE
- CHARACTERISTICS OF PANCREATIC JUICE
- EXTERNAL SECRETION OF PANCREAS
- TESTS IN PANCREATIC DISEASES
- DETERMINATION OF BICARBONATE
- DETERMINATION OF BILIRUBIN (See CHAPTER 30)
- INDIRECT STIMULATION OF PANCREAS
44:CSF (Cerebrospinal Fluid)
- BIOCHEMICAL ANALYSIS
45:Centrifugation
- TYPES OF CENTRIFUGE
- TYPES OF ROTOR
- TYPES OF CENTRIFUGAL SEPARATION
APPENDICES: APPENDICES
APPENDIX 1:Safety in the Clinical Biochemistry Laboratory
- MECHANICAL AND ELECTRICAL HAZARDS
- CHEMICAL HAZARDS
- EXPLOSIVE CHEMICALS
- POISONS
- INFECTION HAZARD
- DISINFECTANTS
- PRECAUTIONS
- FIRST AID MEASURES
APPENDIX 2:Case Reports
APPENDIX 3:Reference Values
- REFERENCE VALUES IN BLOOD
- REFERENCE VALUES IN URINE
- REFERENCE VALUES IN CSF
- REFERENCE VALUES IN GASTRIC JUICE
APPENDIX 4:Anticoagulants
- PRESERVATION OF URINE
APPENDIX 5:Solutions
APPENDIX 6:Preparation of Reagents
APPENDIX 7:Strength and Density of Reagents
- STRENGTH OF SOME COMMON ACIDS AND AMMONIUM HYDROXIDE
- DENSITY OF VARIOUS LIQUIDS

INDEX

TOC
Index

Chapter Notes