Chief Editor Col Pankaj Talwar VSM
Professor Obstetrics & Gynecology and Head of the Department Assisted Reproductive Technologies Center INHS Asvini,
Mumbai, Maharashtra, India
7Contributors
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Manual of Cytogenetics in Reproductive Biology
First Edition: 2014
9789350909928
Printed at
5Dedication
I dedicate this book to my father Mr Mohinder Pal Talwar and mother Mrs Madhu Talwar for their guidance and constant support rendered to me. Such monumental work would not have been possible without their blessings and encouragement. I sincerely thank my wife Dr (Mrs) Neetu Talwar and my children Pratik and Arjun, for giving me unfailing support and encouragement during compilation of the book. It was their unflinching motivation and enthusiasm, which gave me impetus to move ahead in trying times, which I faced during the scripting of the book. I am also grateful to our patients who in many ways have been our best teachers
- Ajit Singh Oberoi
- Research Scholar
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Apoorva Kulshreshtha
- Senior Resident
- Department of Obstetrics and Gynecology
- Employees' State Insurance-Postgraduate Institute of Medical Sciences and Research (ESI-PGIMSR)
- New Delhi, India
- Arun Arora
- IVF Specialist and Gynae Endo-Surgeon
- Pushpanjalilife – Centre for Women Health
- Jammu, Jammu and Kashmir, India
- Ashraf CM
- Clinical Director
- CRAFT Hospital and Research Center
- Kodungallur, Kerala, India
- Bindu Mehta
- Clinical Embryologist
- Vaunshdhara Clinic and Assisted Conception Center
- Nagpur, Maharashtra, India
- Kumar BS
- PhD Scholar
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Dinesh Tomar
- Laboratory Technician
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- GD Maiti
- Head
- Department of Obstetrics and Gynecology
- INHS Asvini
- Mumbai, Maharashtra, India
- Geetu Mahajan
- Lecturer
- Acharya Shri Chander College of Medical Sciences
- Jammu, Jammu and Kashmir, India
- Indu Chawla
- Consultant and Associate Professor
- Department of Obstetrics and Gynecology
- Ram Manohar Lohia Hospital
- New Delhi, India
- Jairam Yadav
- Senior Resident
- Department of Obstetrics and Gynecology
- INHS Asvini
- Mumbai, Maharashtra, India
- Jhumur Pani
- Research Scholar
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Koumudi Godbole
- Fellow of Canadian College of Medical Geneticists
- Consultant Clinical Geneticist
- Department of Genetic Medicine
- Deenanath Mangeshkar Hospital and Research Center
- Pune, Maharashtra, India
- Kranthi Vemprala
- Post Doctoral Fellow
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Krishan Chugh
- Director and Head
- Department of Pediatrics and Pediatric Intensive
- Care Unit (PICU)
- Fortis Memorial Research Institute
- Gurgaon, Haryana, India
- Kuldeep Jain
- Director
- Kuldeep Jain's IVF and Laparoscopic Centre
- New Delhi, India
- Kuldeep Mohanty
- Research Scholar
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Leena Wadhwa
- Associate Professor
- Department of Obstetrics and Gynecology
- Employees' State Insurance–Postgraduate Institute of
- Medical Sciences and Research (ESI–PGIMSR)
- New Delhi, India
- Manisha Vajpayee
- Scientific Director
- Centre of Reproductive Medicine and Genetics
- GBH American Hospital
- Udaipur, Rajasthan, India
- Manoj Kumar
- PhD Student
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Monika Gupta
- Assistant Professor
- Department of Obstetrics and Gynecology
- Hamdard Institute of Medical Sciences and Research and HAHC Hospital
- New Delhi, India
- MS Ahuja
- Professor
- Department of Anatomy
- Armed Forces Medical College
- Pune, Maharashtra, India
- Natachandra Chimote
- Consultant Clinical Embryologist and Scientific Director
- Vaunshdhara Clinic and Assisted Conception Center
- Nagpur, Maharashtra, India
- Neetu Talwar
- Senior Consultant
- Division of Pediatrics Pulmonology
- Fortis Memorial Research Institute
- Gurgaon, Haryana, India
- Nikhil Moorchung
- Senior Advisor Molecular Biology
- Armed Forces Medical College
- Pune, Maharashtra, India
- Nishad Chimote
- Laboratory Director
- Vaunshdhara Clinic and Assisted Conception Center
- Nagpur, Maharashtra, India
- Col Pankaj Talwar VSM
- Professor
- Obstetrics and Gynecology
- Head of the Department
- Assisted Reproductive Technologies Center
- INHS Asvini, Mumbai, Maharashtra, India
- Prajnya Ranganath
- Assistant Professor and Head
- Department of Medical Genetics
- Nizam's Institute of Medical Sciences, Hyderabad
- Adjunct Scientist
- Diagnostic Division
- Center for DNA Fingerprinting and Diagnostics
- Hyderabad, Andhra Pradesh, India
- Col Prasad Lele
- Senior Advisor
- Department of Obstetrics and Gynecology
- Reproductive Medicine Specialist
- Command Hospital (Southern Command)
- Pune, Maharashtra, India
- Rajvi H Mehta
- Director (Academics, Research and Training)
- Trivector Embryology Support Academy
- Mumbai, Maharashtra, India
- Raman Agrawal
- Department of Translational Pulmonology
- Translational Lung Research Center (TLRC)
- Heidelberg University, Germany
- Rashmi Malik
- Specialist
- Department of Obstetrics and Gynecology
- University College of Medical Sciences and Guru Teg Bahadur Hospital
- New Delhi, India
- Rashmi Sharma
- Director
- Origyn Fertility and IVF
- Department of Obstetrics and Gynecology
- Max Hospital
- New Delhi, India
- Rima Dada
- Professor
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Sarabpreet Singh
- Consultant
- Department of Reproductive Medicine
- Artemis Health Institute
- Gurgaon, Haryana, India
- Monis Bilal Shamsi
- Research Scholar
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Sheila S Mathai
- Professor and Head
- Department of Pediatrics
- Armed Forces Medical College
- Pune, Maharashtra, India
- Sohani Verma
- Senior Consultant
- Obstetrician, Gynecologist, Infertility and IVF Specialist
- In-charge IVF Unit
- Indraprastha Apollo Hospitals
- New Delhi, India
- SR Das
- Senior Advisor and Professor
- Department of Pediatrics and Clinical Hematology
- INHS Asvini
- Mumbai, Maharashtra, India
- Sujatha R
- Scientific Director
- CRAFT Hospital and Research Center
- Kodungallur, Kerala, India
- Surveen Ghumman Sindhu
- Senior Consultant
- IVF and Reproductive Medicine Unit
- Max Hospital
- New Delhi, India
- Swetasmita Mishra
- Research Scholar
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Tarranum Hassan
- PhD Scholar
- Laboratory of Molecular Reproduction and Genetics
- Department of Anatomy
- All India Institute of Medical Sciences
- New Delhi, India
- Vijayakumar Chelur Narayanamurthy
- Chief Embryologist
- Embryology Academy for Research and Training
- Mumbai, Maharashtra, India
- Col Yoginder Singh
- Professor and Head
- Fetal Medicine
- Department of Obstetrics and Gynecology
- Command Hospital
- Lucknow, Uttar Pradesh, India
The field of reproductive medicine and clinical embryology has expanded over the past few years. With the advancements in the field of assisted reproductive technologies, our understanding of the reproductive medicine has progressed manifold.
Along with this increase in the knowledge of basic reproductive sciences and pioneering work being done in this field, advanced equipment and culture media have been developed. Today, molecular cytogenetics plays an important role in the outcome of assisted reproductive technologies and has presently become an essential diagnostic tool for the reproductive biologists, medical geneticists, genetic counselors, pediatricians, gynecologists, fetomaternal specialists and oncologists. Traditional cytogenetics has now been amalgamated with the DNA analysis. This is often referred to as “molecular cytogenetics” and represents the single most significant advance in this field in decades and is considered as a fundamental part of the emblematic cytogenetics.
Manual of Cytogenetics in Reproductive Biology is divided into six sections. The first section provides the historical perspective and explanation of the concepts involved in molecular cytogenetics including a detailed description of cytogenetic nomenclature. The section also covers the basics of microscopy and cell structure to simplify the further discussions. The second section is an overview of the various investigative technologies. Peripheral karyotyping and polymerase chain reaction (PCR) techniques are highlighted for easy understanding of the busy reproductive biologists. The purpose of this section is to provide a fundamental understanding of the basic molecular-cytogenetic techniques. The third section comprises the main focus of the book and covers nearly all the burning issues in our field as pertaining to the human chromosomes. The fourth section covers various aspects of recent advances in cytogenetics. Various recombinant technologies and essential aspects of gene therapy are well documented for the readers. The fifth section covers the essential cytogenetics as applied to the field of preimplantation genetic diagnosis. The final section deals with the prenatal cytogenetics and covers various aspects of the early fetal diagnosis.
I gratefully acknowledge the hard work by my colleagues who have authored various chapters of the book. I also thank our publisher for supporting this academic effort of our team.
Please feel free to communicate with us, if you need any clarifications or have any suggestions.
Col Pankaj Talwar VSM
13Acknowledgments
- I will always be grateful to Surgeon Rear Admiral Sushil Kumar NM, VSM for his valuable input and constant motivation. I also sincerely thank my energetic colleague Bhavni Oberoi, for proofreading, typesetting and formatting of the book and Rana Bhimsen, Munendra Singh, and Rakesh Tahbildar, for compilation the voluminous text.
- We would like to thank all the contributors for their valuable inputs and the team at M/s Jaypee Brothers Medical Publishers (P) Ltd, New Delhi, India, for bringing this book to its present form.