Practical Manual of Histology for Medical Students Neelkanth Kote
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1Introduction2

Color Atlas with Identification PointsCHAPTER 1

  1. Loose areolar connective tissue (Fig. 6.12A)
    • Long wavy collagen fibers
    • Short branching elastic fibers
    • Different types of connective tissue cells.
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  2. Adipose tissue (Fig. 6.13A)
    • Rounded/Oval/Polygonal cells
    • Lobules with interlobular septum
    • Signet ring appearance.
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  3. Compact bone [Transverse section (TS)] (Fig. 7.2A)
    • Presence of haversian system
    • Haversian canal containing blood vessels
    • Different types of lamellae.
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  4. Compact bone [Longitudinal section (LS)] (Fig. 7.3A)
    • Longitudinal sections of haversian canal
    • Horizontal sections of Volk mann's canal
    • Lamellae parallel to haversian canal.
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  5. Hyaline cartilage (Fig. 7.7A)
    • Outermost perichondrium
    • Homogeneous matrix
    • Chondrocytes forming cell nests.
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  6. Elastic cartilage (Fig. 7.8A)
    • Outermost perichondrium
    • Matrix rich in elastic fibers
    • Numerous chondrocytes in matrix.
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  7. White fibrocartilage (Fig. 7.9A)
    • Absence of perichondrium
    • Matrix rich in collagen fibers
    • Very few chondrocytes arranged in rows.
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  8. Skeletal muscle (LS) (Fig. 8.2A)
    • Long cylindrical muscle cells
    • Alternative dark and light bands
    • Multinuclei at the periphery.
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  9. Skeletal muscle (TS) (Fig. 8.3A)
    • Peripherally nucleated muscle bundles or fascicles
    • Perimysium around the muscle fascicles
    • Epimysium covering the whole muscle tissue.
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  10. Smooth muscle (LS) (Fig. 8.4)
    • Long spindle-shaped myocytes in bundle
    • Absence of striations
    • Central flat oval nucleus.
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  11. Cardiac muscle (LS) (Fig. 8.5A)
    • Long cylindrical muscle fibers
    • Centrally place nucleus
    • Presence of intercalated disks.
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  12. Peripheral nerve (LS) (Fig. 9.3A)
    • Long cylindrical nerve fibers
    • Central axon with myelin sheath
    • Presence of nodes of Ranvier.
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  13. Peripheral nerve (TS) (Fig. 9.4A)
    • Nerve fibers arranged in fascicles
    • Perineurium covering the fascicles
    • Epineurium covering the whole nerve.
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  14. Spinal ganglion (Fig. 9.5)
    • Large pseudounipolar neurons
    • Neurons with centrally placed nuclei
    • Well-described satellite cells.
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  15. Sympathetic ganglion (Fig. 9.6)
    • Small multipolar neurons
    • Neurons with eccentrically placed nuclei
    • Poorly defined satellite cells.
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  16. Lymph node (Fig. 10.1A)
    • Subcapsular sinus or space
    • Cortex showing lymphatic nodules
    • Medulla rich in blood vessels.
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  17. Spleen (Fig. 10.2A)
    • Outermost capsule with trabeculae
    • White pulp with eccentrically placed arteriole
    • Red pulp rich in blood sinusoids.
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  18. Thymus (Fig. 10.3A)
    • Outermost capsule with incomplete septae
    • Lobules with cortex and medulla
    • Hassall's corpuscles in medulla.
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  19. Palatine tonsil (Fig. 10.4A)
    • Deep tonsillar crypts
    • Stratified squamous non-keratinized epithelium lining the surface
    • Lymphatic nodules with germinal center.
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  20. Large artery (Fig. 11.2A)
    • Circular lumen
    • Thick tunica media rich in elastic fibers
    • Vasa vasorum in tunica adventitia.
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  21. Medium size artery (Fig. 11.3A)
    • Circular lumen
    • Prominent internal elastic lamina
    • Tunica media rich in circular smooth muscles.
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  22. Large size vein (Fig. 11.6A)
    • Collapsed lumen
    • Thin tunica media with circular muscle fibers
    • Thick tunica adventitia with longitudinal smooth muscles and vasa vasorum.
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  23. Medium size vein (Fig. 11.7A)
    • Collapsed lumen
    • Tunica media containing circular smooth muscles
    • Tunica adventitia with dense irregular connective tissue.
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  24. Thick skin (Fig. 12.3A)
    • Thick epidermis of stratified squamous keratinized epithelium
    • Prominent dermal and epidermal papillae
    • Absence of hair follicles.
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  25. Thin skin (Fig. 12.4A)
    • Thin epidermis of stratified squamous non-keratinized epithelium
    • Unmarked dermal and epidermal papillae
    • Hair follicles with sebaceous glands and arrector pili muscles.
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  26. Circumvallate papilla (Fig. 13.2A)
    • Lined by stratified squamous non-keratinized epithelium
    • Numerous taste buds in the groove
    • Presence of serous glands of von Ebner.
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  27. Filiform papilla (Fig. 13.3A)
    • Finger-shaped structure
    • Lined by stratified squamous keratinized epithelium
    • Skeletal muscle protruding its substance.
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  28. Fungiform papilla (Fig. 13.4A)
    • Mushroom-shaped structure
    • Lined by stratified squamous non-keratinized epithelium
    • Skeletal muscle protruding its substance.
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  29. Parotid salivary gland (Fig. 14.2A)
    • Serous acini with narrow lumen
    • Centrally placed round nuclei
    • Numerous ducts are present.
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  30. Sublingual salivary gland (Fig. 14.3A)
    • Mucous acini with a large lumen
    • Flat nucleus placed near the base of the cell
    • Very few ducts are present.
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  31. Submandibular salivary gland (Fig. 14.4A)
    • Both serous and mucous acini are seen
    • Serous demilunes of Gianuzzi are present
    • Few ducts are present.
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  32. Esophagus (TS) (Fig. 15.1A)
    • Mucosa lined by stratified squamous non-keratinized epithelium
    • Submucosa containing esophageal glands
    • Muscularis externa containing inner circular and outer longitudinal muscle layer.
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  33. Stomach (Fundus) (TS) (Fig. 15.2A)
    • Mucosa lined by simple columnar epithelium
    • Numerous chief and parietal cells in lamina propria
    • Muscularis externa containing inner oblique, middle circular and outer longitudinal muscle layer.
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  34. Stomach (Pylorus) (TS) (Fig. 15.3A)
    • Deep gastric pits
    • Lamina propria containing pyloric glands
    • Thickened middle circular muscle layer forming pyloric sphincter.
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  35. Duodenum (TS) (Fig. 16.2A)
    • Numerous villi
    • Crypts of Lieberkühn present
    • Brunner's glands in submucosa.
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  36. Jejunum (TS) (Fig. 16.3A)
    • Tall villi lined by simple columnar epithelium with goblet cells
    • Absence of Brunner's glands in submucosa
    • Absence of Peyer's patches.
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  37. Ileum (TS) (Fig. 16.4A)
    • Small villi rich in goblet cells
    • Crypts of Lieberkühn present
    • Mucosa containing Peyer's patches.
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  38. Vermiform appendix (TS) (Fig. 16.5A)
    • Villi are absent
    • Lamina propria containing lymphoid aggregations
    • Thick muscular layer.
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  39. Colon (TS) (Fig. 16.6A)
    • Mucosa with folds, but no villi
    • Numerous goblets cells
    • Prominent teniae coli.
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  40. Pancreas (Fig. 16.7A)
    • Numerous serous acini
    • Islets of Langerhans are present
    • Interlobular ducts are seen.
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  41. Liver (Fig. 17.1A)
    • Hepatic lobules with central vein
    • Radiating hepatic cord cells
    • Portal triad is seen.
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  42. Gallbladder (Fig. 17.4A)
    • Honeycomb appearance of mucosa
    • Absence of submucosa and muscularis mucosa
    • Presence of fibromuscular coat.
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  43. Kidney (Fig. 18.2A)
    • Outer cortex and inner medulla
    • Cortex containing renal corpuscles, proximal and distal convoluted tubules
    • Medulla containing Henle's loop and collecting ducts.
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  44. Ureter (TS) (Fig. 18.4A)
    • Star-shaped lumen
    • Mucosa lined by transitional epithelium
    • Inner longitudinal and outer circular muscle coat present.
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  45. Urinary bladder (TS) (Fig. 18.5A)
    • Mucosa lined by transitional epithelium
    • Submucosa is absent
    • Muscular layer containing detrusor muscles.
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  46. Trachea (TS) (Fig. 19.2A)
    • Mucosa lined by pseudostratified ciliated columnar epithelium with goblet cells
    • Submucosa containing serous and mucous glands
    • Hyaline cartilaginous ring.
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  47. Lung (Fig. 19.3A)
    • Honeycomb appearance of alveoli
    • Simple squamous epithelium lining the pulmonary alveoli
    • Intrapulmonary bronchi, bronchioles and respiratory bronchioles are seen.
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  48. Pituitary gland (Fig. 20.1A)
    • Pars anterior rich in cells and sinusoids
    • Interglandular cleft with few colloidal vesicles
    • Pars posterior rich in pituicytes and nerve fibers.
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  49. Thyroid gland (Fig. 20.4A)
    • Thyroid follicles lined by cuboidal epithelium
    • Eosinophilic colloid in follicular cavity
    • Parafollicular cells are seen.
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  50. Parathyroid gland (Fig. 20.5A)
    • Irregular cords of chief cells
    • Large eosinophilic oxyphil cells
    • Thyroid gland may be the accompanying structure.
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  51. Suprarenal gland (Fig. 20.7A)
    • Outermost capsule with trabeculae
    • Cortex showing zona glomerulosa, fasiculata and reticularis
    • Medulla showing sinusoids and sympathetic ganglion cells.
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  52. Testis (Fig. 21.2A)
    • Numerous seminiferous tubules
    • Interstitial cells of Leydig present
    • Sertoli cells in seminiferous epithelium.
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  53. Epididymis (TS) (Fig. 21.5A)
    • Tubules lined by pseudostratified columnar epithelium
    • Sperms in the tubular lumen
    • Thin circular muscle layer surrounding the ducts.
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  54. Vas deferens (TS) (Fig. 21.6A)
    • Small stellate lumen
    • Lined by pseudostratified ciliated columnar epithelium
    • Thick muscle coat of inner longitudinal, middle circular and outer longitudinal layer.
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  55. Seminal vesicle (Fig. 21.8A)
    • Branching and anastomosing mucosal folds
    • Mucosa lined by pseudostratified columnar epithelium
    • Muscular coat of inner circular muscle and outer longitudinal muscles.
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  56. Prostate (Fig. 21.9A)
    • Prostatic acini, lined by simple columnar epithelium
    • Fibromuscular stroma
    • Corpora amylacea in the lumen.
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  57. Ovary (Fig. 22.2A)
    • Tunica albuginea with germinal epithelium
    • Cortex showing ovarian follicles of different stages
    • Medulla showing blood vessels.
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  58. Fallopian tube (TS) (Fig. 22.4A)
    • Highly folded mucosa
    • Mucosa lined by simple ciliated columnar epithelium
    • Inner circular and outer longitudinal muscle coat.
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  59. Uterus (Proliferative phase) (Fig. 22.5A)
    • Endometrium lined by simple columnar epithelium
    • Lamina propria containing uterine glands
    • Thick myometrium with numerous blood vessels.
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  60. Cerebrum (Fig. 23.2A)
    • Outer pia mater
    • Six layered cells are present
    • Inner and outer bands of Baillarger are visible.
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  61. Cerebellum (Fig. 23.3A)
    • Outer molecular layer
    • Middle Purkinje cell layer
    • Inner granular layer.
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  62. Spinal cord (Fig. 23.4)
    • Outer white matter
    • Inner H-shaped gray matter
    • Central canal is present.
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  63. Cornea (Fig. 24.1A)
    • Outer stratified squamous non-keratinized epithelium
    • Intermediate stroma
    • Inner endothelial lining.
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  64. Eyeball (Retina) (Fig. 24.2A)
    • Outermost pigment cell layer
    • Middle plexiform and nuclear layer
    • Innermost internal limiting membrane.
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  65. Tooth (LS) (Fig. 13.1)
    • Outermost enamel
    • Prominent dentin with dentinal tubules
    • Dental pulp containing blood vessels and nerve fibers in it.
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  66. Uterus (Secretory phase) (Fig. 22.6)
    • Thick endometrium lined by simple columnar epithelium
    • Lamina propria containing tortuous glands and coiled arteries
    • Myometrium with numerous blood vessels.
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  67. Mammary gland (Lactating) (Fig. 22.7)
    • Numerous prominent acinar lobules
    • Acinar lobules lined by simple columnar epithelium
    • Minimal connective tissue with duct system.
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  68. Umbilical cord (Fig. 22.8)
    • Two umbilical arteries
    • One umbilical vein
    • Wharton's jelly is present.
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