Principles & Interpretation of Laboratory Practices in Surgical Pathology Shameem Shariff, Amrit Kaur Kaler
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fm1 Principles and Interpretation of LABORATORY PRACTICES IN SURGICAL PATHOLOGYfm2
fm3 Principles and Interpretation of LABORATORY PRACTICES IN SURGICAL PATHOLOGY
Shameem Shariff MD PhD Professor and Head Department of Pathology Head Department of Central Research MVJ Medical College and Research Hospital, Bengaluru Rural, Karnataka, India Co-author Amrit Kaur Kaler MD Associate Professor ACS Medical College and Hospital Chennai, Tamil Nadu, India
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Principles and Interpretation of Laboratory Practices in Surgical Pathology
First Edition: 2016
9789352500246
Printed at
fm5Dedicated to
Our families, who endured with patience our time and efforts, Our trainees who will advance knowledge further, Seekers of expertise, whose motivation results in innovations fm6
fm7Preface
The laboratory has been given a central and distinctive role in science education; science educators have suggested that there are rich benefits in learning the accrue from using laboratory activities. Tissues from the body, taken for diagnosis of disease processes, must be processed in the histology laboratory to produce microscopic slides that are viewed under the microscope by pathologists. The techniques for processing the tissues, whether biopsies, larger specimens removed at surgery, or tissues from autopsy, are varied and the persons who do the tissue processing and make the glass microscopic slides are histotechnologists.
While pathologists and cytologists are mainly involved in reporting and interpretation of slides, if the standard criteria for slide preparation are not adhered, this makes the interpretation extremely difficult. As diagnostic personnel, pathologists and cytologists should be aware of all techniques used in the laboratory and have a sound knowledge of the fallacies involved in the preparation. The knowledge of the techniques, which includes processing of specimens, fluids and preparation of these so as to enable them to be transferred to slide material, is all part of the training program for pathologists.
The present book deals exhaustively with methods of processing of tissue including fixation, embedding and cutting, staining techniques and an extensive know-how to combat fallacies in these. The recent techniques with regard to molecular pathology are also dealt with in detail. In addition, certain principles of interpretation are also outlined. In fact, the entire book is user- friendly manual for pathologists, cytologists, cyto- and histotechnologists as well as postgraduate students. It has incorporated our vast experience in cyto- and histopathology.
Shameem Shariff
Amrit Kaur Kaler fm8
fm9Acknowledgments
Our sincere thanks to Dr MJ Mohan, Chairman, MVJ Medical College & Research Hospital, Bengaluru Rural, Karnataka, India, for providing the facilities, atmosphere and environment at the institution, which has enabled us to work towards the completion of this project.
Our sincere thanks to our families whose patience has enabled this book to see the light of the day.
Our thanks are also due to our trainees Dr Pritha Aggarwal, Dr Hasrath Mohammedi and Dr Rajeshwari G, whose efforts in the completion of the compilation are acknowledged.
We are also thankful to Shri Jitendar P Vij (Group Chairman), Mr Ankit Vij (Group President), Mr Tarun Duneja (Director–Publishing) and staff of Bengaluru Production Unit/Bengaluru Branch of M/s Jaypee Brothers Medical Publishers (P) Ltd, New Delhi, for their support.