Manual of Practical Medical Biochemistry Evangeline Jones
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1Manual of Practical MEDICAL BIOCHEMISTRY2
3Manual of Practical MEDICAL BIOCHEMISTRY
Second Edition
Evangeline Jones MD Professor and Head Department of Biochemistry Vinayaka Missions Kirupananda Variyar Medical College Salem, Tamil Nadu, India Formerly, Professor and Head Department of Biochemistry Government Mohan Kumaramangalam Medical College Salem, Tamil Nadu, India
4
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© 2016, Jaypee Brothers Medical Publishers
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Medical knowledge and practice change constantly. This book is designed to provide accurate, authoritative information about the subject matter in question. However, readers are advised to check the most current information available on procedures included and check information from the manufacturer of each product to be administered, to verify the recommended dose, formula, method and duration of administration, adverse effects and contraindications. It is the responsibility of the practitioner to take all appropriate safety precautions. Neither the publisher nor the author(s)/editor(s) assume any liability for any injury and/or damage to persons or property arising from or related to use of material in this book.
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Manual of Practical Medical Biochemistry
First Edition: 2011
Second Edition: 2016
9789352501540
Printed at
5Dedicated to
The memory of my beloved parents
Mr TV Balraj and Mrs Flora Balraj
and
My loving brother Mr BTC Jeyakumar6
7Preface to the Second Edition
The laboratory manual in biochemistry is based on the practical work schedule allotted to the first year MBBS students as per the guidelines issued by the Medical Council of India (MCI).
This book Manual of Practical Medical Biochemistry has been compiled in simple language with a clear focus in equipping the medical students, the clinical aspects of practical biochemistry. This revised second edition has become necessary in view of the changing trends in the teaching and examination patterns of various medical universities. Hence, this book has undergone subtle changes throughout with notable additions being clinical case exercises and objective structured practical examination (OSPE).
I would like to acknowledge with gratitude the help rendered by all the staff of the Department of Biochemistry, Government Mohan Kumaramangalam Medical College, Salem, Tamil Nadu, India, in bringing out the manual, especially my erstwhile colleagues in the Department; Dr R Nagendran MD and Dr Priya Jeyapal MD—Professors of Biochemistry, for their constructive suggestions, and Mrs CR Jamuna BSc, for her help in preparing and typing the manuscript. I also thank all the teaching staff of the Department of Biochemistry, Vinayaka Missions Kirupananda Variyar Medical College, Salem, who helped me to update this edition.
I thank my husband Dr P Jones Ronald MD DM, Nephrologist, for his continuous encouragement and contribution by way of drawing the diagrams in this manual. I thank Shri Jitendar P Vij (Group Chairman), Mr Ankit Vij (Group President) and Mr Tarun Duneja (Director–Publishing) of M/s Jaypee Brothers Medical Publishers (P) Ltd, New Delhi, India, for bringing up this book tidily and on time.
I hope and wish that this manual will be useful for the students of medical, dental and allied medical sciences in understanding the beauty that underlies the basis of biochemistry.
Evangeline Jones8
9Preface to the First Edition
The laboratory manual in biochemistry is based on the practical work schedule allotted to the first year MBBS students as per the guidelines issued by the Medical Council of India (MCI).
This book Manual of Practical Medical Biochemistry has been compiled in simple language with a clear focus on equipping the medical students, the clinical aspects of practical biochemistry.
I would like to acknowledge with gratitude the help rendered by all the staff of the Department of Biochemistry, Government Mohan Kumaramangalam Medical College, Salem, Tamil Nadu, India, in bringing out the manual, especially my erstwhile colleagues in the Department; Dr R Nagendran MD and Dr Priya Jeyapal MD—Professors of Biochemistry, for their constructive suggestions, and Mrs CR Jamuna BSc, for her help in preparing and typing the manuscript. I thank my husband Dr P Jones Ronald MD DM, Nephrologist, for his continuous encouragement and contribution by way of drawing the diagrams in this manual.
I hope and wish that this manual will be useful for the students of medical, dental and allied medical sciences in understanding the beauty that underlies the basis of biochemistry.
Evangeline Jones10
11Acknowledgments
Assistance rendered by Dr R Nagendran MD, Professor, Department of Biochemistry; Dr Priya Jeyapal MD, Professor, Department of Biochemistry; Dr K Anandhi MBBS DO, Tutor, Department of Biochemistry, and Mrs CR Jamuna BSc, Non-Medical Demonstrator, my erstwhile colleagues in the Department of Biochemistry, Government Mohan Kumaramangalam Medical College, Salem, Tamil Nadu, is gratefully acknowledged. I would also like to acknowledge the help extended to me by Dr Vijaya Samundeeswari, MD and Mrs G Ramani, MSc of the Department of Biochemistry, Vinayaka Missions Kirupananda Variyar Medical College, Salem, Tamil Nadu, India.1213141516
17Biochemistry Practicals General Instructions to the Students
  1. All the students should be punctual in attending practical classes. All should wear neat white coat. Dresses made by inflammable materials should be avoided.
  2. All should attend the instruction or demonstration classes before each practical class.
  3. Students can leave the laboratory only after finishing their practicals and getting signature in the observation book.
  4. All should maintain a biochemistry record notebook prescribed by the college and submit them within the stipulated time. Separate marks are allotted for records as per the instruction of the university.
  5. All the glasswares should be cleaned thoroughly and all waste materials should be dropped into the waste basket.
  6. After using, all the reagent bottles should be closed with stoppers and kept in the appropriate places in the racks.
  7. While taking the bottles from the rack, students should hold the bottles with both hands. Should not carry the bottles by the neck.
  8. Pipetting by mouth should be done carefully. Students can use teats whenever necessary. Should not pipette concentrated acids, alkali or toxic chemicals by mouth.
  9. All burners should be put off after the practical work is over.
  10. In the event of any accident, such as spilling of acid or alkali over the body or eye, accidental entry of acid/alkali into the mouth during pipetting, electric shock, gas leakage, burns, etc., it should be informed immediately to the staff for the first aid and emergency treatments.