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Chapter-97 Blastocyst Culture and Transfer

BOOK TITLE: Principles and Practice of Assisted Reproductive Technology

Author
1. Mehta Rajvi H
2. Jaffar Mir
3. C Mohammed Ashraf
ISBN
9789352705030
DOI
10.5005/jp/books/18020_98
Edition
2/e
Publishing Year
2019
Pages
10
Author Affiliations
1. Hope Infertility Clinic Research Foundation, Bangalore, India, Trivector Embryo Support Academy, Mumbai, Maharashtra, India, Support Academy, Mumbai, Maharashtra, India, Trivector Embryology Support Academy, Mumbai, Maharashtra, India, Trivector Origio Pvt Ltd, Mumbai, Maharashtra, India, Trivector Embryology Support Academy, Bengaluru, Karnataka, India, Research and Training, Trivector Origio Pvt Ltd, Mumbai, Maharashtra, India, Research and Training Trivector Origio, Mumbai, Maharashtra, India
2. Milann—The Fertility Center (A Unit of BACC Healthcare Pvt Ltd), Bengaluru, Karnataka, India, Milann—The Fertility Center, Bengaluru, Karnataka, India, Valley Fertility Center, Srinagar, Jammu and Kashmir, India
3. Milann—The Fertility Center, Bengaluru, Karnataka, India, Milann Fertility Centre, Bengaluru, Karnataka, India, Milann Fertility Center, Bengaluru, Karnataka, India
Chapter keywords
Blastocyst culture, blastocyst transfer, culturing blastocyst, in vitro fertilization, zygote, embryos, hatching phenomenon

Abstract

Blastocyst culture, although a little time consuming and its inherent limitations, is an important step ahead in the field of ARTs. However, before it can be routinely applied in an ART laboratory, it is essential that the laboratory first has the requisite infrastructure, maintenance and skills. Although, the pregnancy rate per blastocyst transfer is higher than pregnancy rates following cleavage stage transfer but if one were to look at the cumulative pregnancy rate per cycle started or per patient, then the results are not that dramatic. Because, the number of embryos that grow to blastocyst and are available for transfer or cryopreservation much lesser. This chapter covers the culturing blastocysts in vitro, morphological evaluation of blastocysts, morphology of poor quality blastocysts, evaluation of zygotes and embryos and their potential to develop into blastocysts, factors influencing blastocyst development, hatching phenomenon in a blastocyst, advantages and applications of blastocyst culture, and limitations of blastocyst transfer.

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