Practical Hematology for BDS Komal Marwaha
INDEX
A
ABO blood grouping 84
Acetone 59
Acid citrate dextrose (ACD) 22
Acidified glycerol lysis test (AGLT) 122
Advantages of
Sahli's method 75
Westergren method 112
Wintrobe method 106, 114
ZSR method 115
Anemia 42
classification based on blood indices 108
Assessment of stained blood smear 60
Autologous transfusion 87
Automated method 103
B
Bart's Hb 76
Bleeding
disorders 94
time 90, 124
Blood
determination 87
indices 124
transfusion 87
BT tests 94
C
Calculation of
dilution factor 41, 49
RBC count 41
volume of fluid examined 41, 49
Capillary blood sample 11
Causes of
decrease in hemoglobin concentration 78
increase in hemoglobin concentration 78
Cell volume 103
Centrifugal method 58
Charging chamber 32
Citrate phosphate dextrose-adenine 22
Clinical significance of ESR 117
Clinically important blood group systems 86
Clotting time 90, 124
Coagulation defects 94
Collection of venous blood 14, 15
Composition of Hayem's fluid 39
Copper sulphate falling drop method 77
Counting
chamber 29
cells 41, 48
Cover slip method 57
CT tests formation of clot 96
Cytoplasm 64
to nucleus ratio 64
D
Decrease in HCT 108
Decreased
osmotic fragility 123
RBC count 43
Derivatives of hemoglobin 75
Determination of
BT 90
CT 92
erythrocyte sedimentation rate (ESR) 111
hemoglobin concentration in blood 72
osmotic fragility of RBCs 120
Diagnostic use of ESR 117
Dicumarol 25
Different types of microscopes 8
Differential leukocyte count 67, 124
Diluting pipettes 28
Disadvantages of
Sahli's method 75
ZSR method 115
Disposable syringe 14
Distribution of cells in smear 64
Double oxalate 23
Duke's method 90
E
Ear lobe puncture 13
Errors of hemocytometry 33
Erythrocyte sedimentation rate 124
Estimate of iron content 77
Ethylenediaminetetraacetic acid sequestrene/versene 21
Examination of
peripheral blood smear 55
stained smear under oil immersion 60
F
False
negative results 85
positive results 85
Fate of WBC in RBC count 43
Favouring factors 116
Features of
ideal blood film 57
well-stained blood smear 60
Fixing smear 59
Focusing counting grid 31
Functions of
constituents of Turk's fluid 47
hemoglobin 75
G
Gasometric method 77
Giving false
high value 75
low value 75
Glass slide method 55
Granules 60
H
Heel puncture 13
Hematocrit 103
Hemoglobin concentration 124
Hemoglobinometery 77
Hemoglobinopathies 77
Hemophilia 96
Heparin 20, 24
I
Identification of
cells 60
leukocytes 61
Improved Neubauer chamber 30
In vitro anticoagulants 20
In vivo anticoagulants 24
Increase in HCT 108
Increased RBC count 43
Indications of determining BT and CT 94
Inherent errors 34, 50
Ivy's method 91
L
Landsteiner's law 86
Lee and White test tube method 93
Leukemia 51
Leukemoid reaction 51
Leukocyte pool in body 50
Leukocytosis 50
Leukopenia 51
Leukostasis 51
Lobes 64
M
Major cross matching 87
Manual method 103
Method of
determination
of blood group 82, 103
using microscope 7
Micro-ESR 115
Microhematocrit method 106
Minor cross matching 87
MNS and P system 87
Morphological classification of anemias 107
N
Naked eye
appearance 60
single tube red cell osmotic fragility test 122
Normal
count 50
differential leukocyte count 68
hematological values 124
range of RBCs 42
Normal value 113, 121
by Wintrobe method 114
Nucleus 64
O
Opposing factors 115
Osmotic fragility 124
test 120
Other
blood groups 87
fetal hemoglobins 76
Oval field of view 8
P
Packed cell volume 124
Par focal system 6
Parts of microscope 4
Pathological
causes of leukocytosis 51
conditions 117
Photoelectric colorimetry 77
Physiological causes of leukocytosis 51
Pink test 122
Pipetting 31, 48
Plasma factors 116
Polycythemia 42
Poor illumination 8
Preparation of peripheral blood smear 55
Prerequisites of blood transfusion 87
Principle of staining procedure 59
Purpura 95
R
RBC
diluting fluid 38
pipette 28
Reasons of false counts 34
Red cell factors 116
Rhesus system 86
Role in ESR 116
S
Sahli's method for determination of Hb 72
Significance of osmotic fragility tests 122
Simplate method 92
Size of cell 60
Slide method of blood group determination 82
Sodium
citrate 21
fluoride 24
Spectrophotometric method 77
Stages of ESR 115
Staining of peripheral smear 58
Study of
anticoagulants 20
hemocytometry 27
T
Tallquist's method 77
Tests for determination of
bleeding time (BT) 90
clotting time (CT) 92
Thalassemia 76
Total
erythrocyte count 38
leukocyte count 47
RBC count 43
red blood cell count 124
white blood cell count 124
True hematocrit 107
U
Under
high power 60
low power 60
Universal
donor 87
recipient 87
Uses of
HCT determination 107
RBC pipette 28, 43
WBC pipette 29
V
Vacuum blood collection system 15
Variation in WBC count 50
Varieties of Hb 76
Venous blood sample 14
Vessel wall defects 94
von Willebrand disease 95
W
Warfarin 25
Water free methyl alcohol 59
WBC pipette 29
Wedge method 55
Westergren method 111, 124
Whole body HCT 107
Wintrobe method 103, 113, 124
Wright's capillary glass tube method 92
Z
Zeta sedimentation ratio (ZSR) 115
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Chapter Notes

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