fm4 Jaypee Brothers Medical Publishers (P) Ltd.

J.P. Medical Ltd

83 Victoria Street, London

SW1H 0HW (UK)

Phone: +44 20 3170 8910

Fax: +44 (0)20 3008 6180

Email: info@jpmedpub.com

Jaypee Brothers Medical Publishers (P) Ltd

17/1-B Babar Road, Block-B, Shaymali

Mohammadpur, Dhaka-1207

Bangladesh

Mobile: +08801912003485

Email: jaypeedhaka@gmail.com

Jaypee-Highlights Medical Publishers Inc

City of Knowledge, Bld. 235, 2nd Floor, Clayton

Panama City, Panama

Phone: +1 507-301-0496

Fax: +1 507-301-0499

Email: cservice@jphmedical.com

Jaypee Brothers Medical Publishers (P) Ltd

Bhotahity, Kathmandu

Nepal

Phone: +977-9741283608

Email: kathmandu@jaypeebrothers.com

Jaypee Medical Inc

325 Chestnut Street

Suite 412, Philadelphia, PA 19106, USA

Phone: +1 267-519-9789

Email: support@jpmedus.com

The views and opinions expressed in this book are solely those of the original contributor(s)/author(s) and do not necessarily represent those of editor(s) of the book.

All rights reserved. No part of this publication may be reproduced, stored or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior permission in writing of the publishers.

All brand names and product names used in this book are trade names, service marks, trademarks or registered trademarks of their respective owners. The publisher is not associated with any product or vendor mentioned in this book.

Medical knowledge and practice change constantly. This book is designed to provide accurate, authoritative information about the subject matter in question. However, readers are advised to check the most current information available on procedures included and check information from the manufacturer of each product to be administered, to verify the recommended dose, formula, method and duration of administration, adverse effects and contraindications. It is the responsibility of the practitioner to take all appropriate safety precautions. Neither the publisher nor the author(s)/editor(s) assume any liability for any injury and/or damage to persons or property arising from or related to use of material in this book.

This book is sold on the understanding that the publisher is not engaged in providing professional medical services. If such advice or services are required, the services of a competent medical professional should be sought.

Every effort has been made where necessary to contact holders of copyright to obtain permission to reproduce copyright material. If any have been inadvertently overlooked, the publisher will be pleased to make the necessary arrangements at the first opportunity.

Inquiries for bulk sales may be solicited at: jaypee@jaypeebrothers.com

First Edition: 2017

The relentless progresses of medical technology have brought into its wake novel methods of investigations and treatments, rendering many older methods obsolete. Pathological investigations remain cornerstone of medical diagnosis. Histotechnology plays a pivotal role to make a diagnosis of surgical specimens.

With the advent of newer diagnostic techniques like molecular diagnostic methods, a quick but very reliable diagnosis is possible, even from small amount of tissue. In this manual, I have included the molecular techniques, immunohistochemistry, cell blocks, and immunofluorescence along with the conventional techniques. For students' easy understanding; many figures, charts, diagrams and tables have been included. At the same time, the volume of the book has been restricted; so that students do not become overburdened during preparation of examination. Specialists and consultants who are working in a surgical pathology laboratory will also find it useful.

I am indebted to my parents Mr Nitai Chandra Mondal and Smt Jyotsna Mondal for their value-based guidance, blessings and constant support that I have received throughout my life. I thank my son Soumyadeep, brother Monotosh and wife Shampa, for their continuous encouragement during preparation of this manual. I also thank postgraduates of pathology (MD, PGT), especially Dr Saikat Mandal and Dr Debasish Bhattacharya, for their help during preparation of this book. I am thankful to my friend Dr Sanjib Pattari, for supplying a few special stained slides.

Despite my best efforts, some mistakes might have crept in. So, I request all readers to kindly bring it to my notice. Your constructive criticism, appreciation and suggestions are most welcome.