A
ABO
antibody titer 215
blood group 203
system 208
tube technique for 209
gene 208
titer 217t
weaker variants 208
Acanthocytes 5
Acid solution 283
Acidic components 4
Acquired immunodeficiency syndrome 236
Acrylamide stock solution 469
Acute myeloid leukemia, development of 303
Agglutination reaction 208
Agkistrodon contortrix 178
Albumin 223
Aldolase 404
Alloimmunization, prevention of 232
Alpha-thalassemia deletions 64t
Alphorids 288
Amine incorporation assays 98
Ammonia release assay 97
Ampicillin 288
Amplification 263
Amplified fragments, resolution of 140
Anemia, classification of 7t
Anisocytes 5
Antibiotics 288
Antibody 174
defects 340fc
details of 361t
detection 106
iodination of 121
panels 328
quantitation of 264
Anticoagulant 407
Anticoagulation with heparin 178
Anticomplement factor H 197
Anticyclic citrullinated peptide antibodies test 273
Antifade mounting medium 290
Antifade solution 293
Antiflavocytochrome 361
Antiglobulin sera 223
technique, cross-match by indirect 241
Anti-H titers 215
Antihuman monoclonal anti-fetal hemoglobin antibody 403
Antimitochondrial antibodies 272
Antimycobacterial immunity 367
Antiperinuclear factor 273
Antiphospholipid antibodies 167
Antiserum 206
Anti-von Willebrand factor antibody 119
Apoptosis assay 382
Arginine hot spot mutations, screening for 131
Aspergillus species 321
Atrial fibrillation 188
Autoimmune
disorders 265
Autoinflammatory disorders 319
Automated electrophoresis technique 46
Avidin 290
B
B substance 212
Baby's cord blood, tests for 234
Bacteria 321
Bacterial artificial chromosome 287f
Bacterial clone deoxyribonucleic acid mini-preparation 287
Bacterial culture work 310
Bacto yeast extract 288
Bactotrypton 288
Basophilic stippling 5
Beta-mercaptoethanol 290
Bethesda assay graph 105f
Biochemical basis 107
Bisacrolylpiperazine 475
Borate ethylenediaminetetraacetic acid 469
Bothrops atrox 93
Bovine serum albumin 121
Branched deoxyribonucleic acid technology 259
Bromophenol 415
Bursttest test kit 360
C
C3B/C4B inactivator 198
Candida species 321
Capillary electrophoresis 481
Carnoy's fixative 307
CD119 on monocytes, expression of 365
CD4 lymphopenia 326
CD8 lymphopenia 326
Centromere pattern 269
Centrosomic nucleoprotein B 272
Charge-coupled device 293
Chloramphenicol 288
Chloroform 468
Chorionic villus sample 132
Chromophoric group 101
Chromosomal breakage investigation 307
Chromosomal instability syndromes 283
Classical bethesda assay 104
Clauss assay, modified 94
Coagulation cascade 88f
Coated autoantibodies, elution of 239
Coating buffer 106
Colchicine stock solution 286
Cold
agglutinin syndrome 236
antibodies, titration for 239
centrifuge 315
hem-agglutinin disease 236
Column agglutination technique, cross-match by 223
Commercial panel cells 228
Complement control protein 197
conversion procedure 298
master mix components 298t
preparation for 299t
thermal cycler conditions for 299t
Complementary test 147f
Congenital neutropenia, severe 353
Consensus repeats, short 197
Conventional staining 286
Coomassie stain 405
Copy number status dosage quotient 482
Cross-matching procedures 222
Cross-reacting material 102
Cryptococcus neoformans 321
Cryptosporidium parvum 321
Cyanine 289
Cyclic citrullinated peptide 279
Cytochrome B-245 alpha 355
Cytofix 369
Cytomegalovirus 321
Cytometer set-up and tracking 323
Cytoperm 369
Cytoplasmic antineutrophil cytoplasmic antibodies 270
D
Dde1 alleles, resolution of 153f
Delta thalassemia, detection of 66
Denaturing gradient gel electrophoresis 138
Dendritic cells 350
Deoxyadenosine triphosphate 289
Deoxyguanosine triphosphate 289
Deoxyribonuclease 289
Deoxyribonucleic acid 52
antibodies, anti-double stranded 269
denaturation 480
polymerase 289
precipitation of 470
quality of 471
quantity of 471
electropherogram 67f
exon-wise 75
of beta-globin gene 60
protocol 388t
reaction 81t
spectral karyotyping 294
Deoxyribonucleotide triphosphate 289
Deproteinization 470
Dermatomyositis 271
Destaining solution 402
Detection buffer 290
Diagnostic and complementary tests 148t
Diamidino-2-phenylindole 291
Diepoxybutane 306
Diethylaminoethyl 234
Dimension gels, first 176
Dimorphic population 5
Dipotassium hydrogen phosphate 401
Direct coombs test 34
Direct thrombin inhibitors 102
Disposable syringes with needles 285
Disseminated intravascular coagulation 170
Donors blood sample 242
Dot plots 339f
Dot-blot hybridization, reverse 52f
Double negative T lymophocytes, flow cytometric evaluation of 385f
Dual fusion translocation probe 297f
Duffy blood group antigens, genotyping of 254f
Dysfibrinogenemia 93
E
Earl's medium 295
Electropherogram 246f
Electrophoretic buffer 120
Elevated serum globulin 236
Elliptocytes 5
Embden-Meyerhof-Parnas pathway 34
Enteric flora 321
Enteroviruses 321
Epithelial cells 212
Escherichia coli 310
Exon 430
Extragenic marker 140
F
F cell estimation 403
F-10 nutrient mixture 284
Factor assays, different modes of 100t
Factor deficient plasma 408
Factor X gene 157
Family history and pedigree 85
FANC genes mutation analysis 311
FANCD2 immunoblot patterns 309t
F-cell estimation 32
Ferric chloride 402
Fetal blood analysis 28
Fetal calf serum 310
Fetal D antigen 230f
Flow cytometer-based assays 322
osmotic fragility 42
Fluorescence dye 404
Fluorescence histogram 44f
Fluorescence resonance energy transfer 196
Fluorescent fragments, short 306f
Fluorescent in situ hybridization, slides for 291
Fluorescent microscope using different filters 293
Fluorescent-labeled antibodies 110t
Fragment separation 481
Frozen plasma samples, controlled thawing of 87
Frozen serum samples, controlled thawing of 267
FXIII deficiency, detects mild-to-moderate 96
G
Gastrointestinal diseases 321
Gastrointestinal tract 321
G-banded metaphase 294f
General molecular biology protocols 468
Genesplicer 484
Genetic diseases, diagnosis of 283
Giardia lamblia 321
Giemsa-banding technique using trypsin 287
Glacial acetic acid 285
Glass Pasteur pipettes 307
Glasswares, preparation of 283
Globin gene promoters 71
Glomerulonephritis 270
Glucose-6-phosphate 38
activity test 37
activity, calculation of 38
assay reaction composition 38t
gene 74t
screening for 36
Glucose-tris-ethylenediaminetetraacetic acid lysis buffer 288
Glycerolization, procedure of 413
Glycoproteins 110
GP IIB gene 161
GP1BB gene 163
GP9 gene 163
GPIBA gene 163
GPIBB gene 163
GPIIB gene 161
Granular cytoplasmic staining, diffuse 270
H
Haemophilus influenzae 321
Heat denaturation technique 14
Heat elution 237
Heat stability test 14f
Hematocrit 7
Hematology 61t
Hematopoietic stem cell transplantation 319
Hematoxylin 402
A2 28
quantitation of 17
adult 16
analysis 18
C 3
disease 6
mutation, detection of 49t
detection of unstable 402
disorders of 3
beta thalassemia 24f
homozygous 24f
minor 6
trait 23f
electrophoresis 4
H 16
M, spectrophotometric detection of 31
N 31
beta-thalassemia 6
confirmation of 48
heterozygous cord blood sample 30f
solubility test for 10f
tests for unstable 14
unstable 14f
variant, unstable 15
Hemoglobins, abnormal 17
Hemolysis 217
Hemolytic transfusion reactions, investigations of 242
Hepatitis
B
core antigen 262
B E-antigen 264
enzyme-linked immunosorbent assay 264
deoxyribonucleic acid quantitation 263
viral load 259
Heterozygous alpha thalassemia 5
Hexokinase activity 40
Hexose monophosphate pathway 34
Hha1 alleles, resolution of 154f
High performance liquid chromatography 36
Histoplasmosis capsulatum 321
Homogeneous pattern 269
Hot air oven 283
Human albumin 356
Human chromosome preparation 284
Human globulins 219
Human interleukin-7 receptor subunit alpha 334
Human plasma 263
Human splicing finder 484
Hydrochloric acid 197
Hydrochloride 39
Hypochromia 5
Hypotonic solution 286
I
Imidazole buffered saline, preparation of 407
Immunodeficiency
disorders
genotyping of primary 387
molecular analysis of primary 387
screening, primary 325
syndromes 319
Immunoelectrophoresis 175
Immunoglobulin
dissociation of 238
antibody 233t
coated red cells, preparation of 414
levels, low 326
In vitro incubation 9f
Incubated mix reading 103
Inflammation, chronic 352
Inhibition 219
Inhibitor screen test 103
International normalized ratio 89
International System for Chromosome Nomenclature 287
Interstitial lung disease 277
Intracellular staining protocol 362
Intracranial hemorrhage, cause of 165
Intravenous blood 267
Intrinsic immunity defects 364
Intron 22 inversion analysis 144
Inverse protein C resistance technique 144
ISO-osmotic phosphate buffer 401
Isopropanol-Tris buffer 402
ITG3A gene 161f
ITGA2B gene 161f
K
Kahn tubes 232
Kanamycin 288
Karyotype preparation 287
Kell blood group antigens, genotyping of 255f
Kidd blood group antigens, genotyping of 254f
Kidneys 270
Kinetoplast 269
Kleihauer-Betke test 11
L
Laemmli buffer 308
L-asparaginase therapy 170
Leptocytes 5
Ligase-65 479
Ligation reaction 480
Linkage analysis 138
Listeria monocytogenes 321
Lower respiratory tract 321
Lupus nephritis 275
Luria-Bertani medium 288
Lymphadenitis 321
Lymphocyte 343
cell culture, maintenance of 310
function-associated antigen-1 352
identification of 330f
preparation and culture procedure 308
subset report 331f
Lysate preparation 313
Lyse wash-stain-wash 325
M
Macrocytes 5
Macrophage-activation syndrome 375
Macrothrombocytopenia 163
Magnesium chloride 38
Maternal serum antibody 232
Mature human erythrocytes 33
Maxentscan 484
Mean channel fluorescence 44
Membrane protein defects, identification of 46
Membrane-Biodyne C transfer membrane 406
Memory cells 337
Mendelian inheritance 319
Meningitis 321
Mepacrine 112
Metaphase plate 296f
Micro pipettes 292
Microangiopathic hemolytic anemia 196
Microcentrifuge tubes 292
Microcytes 5
Mitomycin C 306
Mitotic index 287
Monoclonal immunoglobulin M 203
Mononuclear cell separation 310
Moraxella catarrhalis 321
Multiple ligation-dependent probe amplification, advantages of 482
Multiple vitamin K dependent coagulation factor deficiency 93
Mycobacteria 321
Mycobacterium tuberculosis 365
Mycoplasma pneumoniae 321
Myocardial infarction 188
N
Naïve T cells 337
Nasal passages 270
Natural anticoagulants protein C 167
Neisseria meningitides 321
Neonatal alloimmune thrombocytopenia 165
Nephelometry-based assays 343
Nestroft test 401
New methylene blue stain 401
Nitroblue tetrazolium test
interpretation of 357
microscopic examination of 357f
negative 357
positive 357
Nonfunctional receptor 367f
Nonsecretor saliva 213
Nontuberculous environmental mycobacteria 365
Normal cord blood sample 29f
Normal healthy control 109f
Nucleolar pattern 269
Nucleotide deletion 73f
Nucleotide polymorphisms, single 482
Nucleotide replacement 261
O
O cells 225
O pooled cells 232
Octanol 468
Oligo probes, blotting of 406
Oligonucleotide probes 406
Omenn syndrome 326
Optical density 113
Osmotic fragility test 42
Otitis 321
Owren's buffer 98
Oxidative burst function 357f
Oxidized glutathione 404
P
Panel cells 412
Partial autosomal dominant form-over expression 367f
Partial thromboplastin 90
Patassium chloride 39
Perforin for hemophagocytic lymphohistiocytosis workup, evaluation of 377t
Perinuclear antineutrophil cytoplasmic antibody 270
Perinuclear staining 270
Peripheral venous blood samples 322
Phenol 468
Phenol-chloroform method 470
Philadelphia chromosome A 294f
Phospho signal transducer and activator of transcription
assay, detection of 368t
Phospholipids 90
Phosphorylated signal transducer 364
Plant lectin 203
Platelet 111f
aggregation
basic pathways of 108
graph 109f
reagents 409
tests 107
dense granules 112
fresh-washed 116
poor plasma 87
receptor studies 110
rich plasma 409
Pneumocystis jiroveci 321
Pneumonia 321
P-nitro aniline 101
Poikilocytes 5
Polyacrylamide stock solution 474
Polychromasia 5
Polychromic staining solution 4
Polyclonal antibody 403
Polygalacturonic acid 404
protocols 132
composition 245t
conditions 314t
first 300t
for hematology 61t
protocol 299
seminested 300
reagent
second 301t
thermal cycler conditions
for first 300t
for second 301t
for sequencing 302t
transcription
analysis, reverse 298f
reverse 313
test, reverse 297
Polymorphic markers 152f
Polymorphic regions 152t
Polymorphism 167
analysis 48
heterozygous for 138
phenotyping 483
strongly-associated 188
weakly-associated 188
Polymyositis 272
Polyphen 483
Polypoidal choroidal vasculopathy 198
Polysaccharide 340
Ponceau S stain 402
Pooled cells, preparation of 410
Postsequencing product cleanup 302
Postsequencing reaction cleanup 477
Power SYBR master mix 316
Precipitating rabbit antiserum 174
Precipitin tubes 232
Pre-warmed hypotonic solution 286
Probes in membrane 53f
Proliferating cell nuclear antigen 272
Promyelocyte arrest 352
Protamine sulfate neutralization 93
analysis 483
and denaturation, dilution of labeled 46
C
activated 177
activity 170
deficiency 170
resistance, activated 177
S
activity 171
Proteinase K 468
Proteolytic enzymes 411
Prothrombin G20210A polymorphism, alleles of 181f
Protozoa 321
Pseudomonas aeruginosa 321
Pulmonary arterial hypertension 277
Purine nucleoside phosphorylase 326
R
Radiation sensitivity test 326
Radiolabeling antibodies 121
Rare donor cells, preservation of 243
Rare phenotype 243
Re mix for exon 14 BSL1 142t
Reaction mixture preparation 314t
Reactive oxygen species 360
Receiver operating characteristic 273
Reciprocal translocation, double 297f
distribution 7
enzymopathy, diagnosis of 36fc
lysis buffer 405
membranopathy, diagnosis of 36fc
parameters 7t
Red cell
agglutination of 205
disorders 34
enzyme assay 35
reagent for 403
detection of 73
membrane 34
disorders 42
membrane protein
defects 48f
membrane protein deficiency 44
flow cytometric analysis of 404
pellet 225
pooled 206
protein defects, identification of 45
suspension, preparation of 42
Renal tubular acidosis, distal 76
Representative gel 179f
Respiratory syncytial virus 321
Respiratory tract 321
Retroviral gene transfection 310
Retroviral production 310
Rh genotyping 231
Rh hemolytic disease 230
Rh phenotypes and genotypes 231t
Rh zygosity 231
RhD antigen 230
RhD gene 251
RhD genotyping test 256f
RhD positive phenotype donors 253f
Rheumatic factor detection and quantification 272
Rheumatoid factor 272
Ribonuclease 288
Ribonucleoprotein 272
Ribosomal phosphoprotein 272
Ristocetin-induced platelet aggregation 112
Rocket immunoelectrophoresis 174
Room temperature 4
Running buffer 119
Running gel 120
Russell viper venom 169
S
Sample buffer 119
Sample dilution buffer 106
Sample preparation 120
Saponin 401
Scleroderma 271
Screening cells 226
Screening techniques 95
Second dimension gels 176
Semi-log graph paper 99f
Sensitizing antibody 219
Sepsis 321
Serological diagnosis 203
Shaker incubator 287
Sickle cell 9f
anemia 6
disease 28
beta thalassemia 23f
D disease 26f
E disease 25f
homozygous 22f
solubility test for 10
trait 22f
Signal transducer 372
Siliconized glass tubes 104
Single strand conformation polymorphism 475
Single-chain precursor 198
Single-nucleotide polymorphism 165
Single-platform method, analysis for 331
Sinusitis 321
Sjögren's syndrome 278
Skin infections 321
Slides, rapid ageing of 291
polyacrylamide gel
reagents, preparation of 405
Solid surface antigen 106
Solubility test 401
Speckled pattern 269
Spectral karyotyping reagents 294
Spectral overlap 324
Spherocytes 5
Splice site prediction softwares 483
Stabilizing solution 403
Stacking buffer 119
Stacking gel 120
Stain-lyse-no wash 325
Stain-lyse-wash 325
Staphylococcus aureus 321
Sterilization procedures 284
Stock Laemmli buffer 405
Stomatocytes 5
Streptavidin-peroxidase 198
Streptococcus pneumoniae 321
Stroke 188
Substrate solution 106
Sulfuric acid 197
Synthetic chromomeric substrate 173
Systemic bacterial infections 321
Systemic viral infections gastroenteritis 321
T
T-helper cells 328
Tag antibody 113
Tandem repeats, variable number of 132
TaqMan assay principle 260
Target cells 5
Tc cells 378
Telomerase detection kit, quantitative 313
Telomerase reaction 314
Telomere standard, sequential dilution of 316t
Test plasma, dilution of 105
Tetramethylbenzidine 122
Tetramethylenediamine 273
Thermal cycling conditions 54t, 57t, 59t, 66t, 68t, 70t, 72t, 74t, 81t, 133t, 135t–137t, 159t–161t, 164t, 182t, 245t, 255t, 299
for intron hind III 140t
Thermal cycling parameters 200t
Thrombocytopenia 196
Thrombotic microangiopathy 196
Thrombotic thrombocytopenic purpura 196
Thymic epithelial cells 350
Thymic stromal lymphopoietin 334
Tissue culture media, preparation of 284
Toxoplasma gondii 321
Transcription 4 activator of 372
Transcription-mediated amplification 259
Transfection 311
Transfusion transmitted disorders 257
Triose-phosphate isomerase 404
Tripotassium citrate 415
Triradial formation 308
Tris-acetate-ethylenediaminetetraacetic acid 469
Trisodium citrate 407
Tuberculosis 365
U
Ultrapure formamide 475
Unstable buffer 402
Upper respiratory tract 321
Urinary tract problems 321
Urokinase plasminogen activator 185
V
Venous thromboembolism 177
Viral ribonucleic acid extraction 262
Viruses 321
cleaving protease 196
collagen binding 118f
determination of 114
gene 123
hot spot mutations of 125t
multimer analysis 119
Vortex tubes 347