Sarode SC, Maniyar N, Sarode GS, Patil S. Abscopal Effect: Propitious or Pernicious? J Contemp Dent Pract 2017;18(5):349-351.

This study evaluated the fluorescence intensity (FI) of different brands of composite resins (CRs) and compare those values with the FI of human tooth, under the action of cigarette smoke (CI), coffee (CA), and soft drink (CO), measured by direct spectrometry.

A total of 30 specimens of each brand (Filtek Z350, Esthet-X, Amelogen, Durafill) were made. Others 30 tooth specimens (3 mm/diameter) were obtained from human molars using a trephine bur. The specimens were randomly divided into three groups (n = 10), according to substances: CI, CA, CO. The FI was directly measured using an optic fiber associated with a spectrometer and was measured at baseline and after staining. Data were submitted to Kruskal–Wallis, Dunnett, and Dunn tests.

Staining influenced FI mean values among CRs and between those with human tooth. Z showed the closest FI mean values of tooth after staining.

Staining beverages and cigarette smoke negatively influenced on FI of CR and human tooth.

The study shows darkening treatments influenced on the fluorescent property of the dental tissues and restorative materials according to the direct spectrometry analysis.

da Silva TM, da Silva NY, Gonçalves LL, Alves LP, Fernandes AU, Gonçalves SEP. Staining Beverages and Cigarette Smoke on Composite Resin and Human Tooth Fluorescence by Direct Spectrometry. J Contemp Dent Pract 2017;18(5):352-357.

The aims of this study were to investigate the prevalence of apical periodontitis (AP) in diabetes mellitus (DM) patients compared with nondiabetic patients and to examine the effect of glycemic control on the prevalence of AP.

Radiographs of a group of DM patients were compared with those of a matched nondiabetic group to identify AP. The diabetic group was subdivided according to the level of glycemic control into two subgroups: A well-controlled DM and a poorly controlled DM. The periapical index score was used to assess the periapical status. All groups were compared in regard to the presence of AP lesions, the number of endodontically treated teeth (ET), and the percentage of failure of endodontically treated teeth (AP/ET ratio). Statistical Package for the Social Sciences (SPSS version 20.0, Chicago, Illinois, USA) was used for all the analyses; p ≤ 0.05 was considered as statistically significant.

The prevalence of AP was higher in diabetic group than in the nondiabetic group (13.5 vs 11.9% respectively). Diabetic group had more teeth with endodontic treatment ET compared with nondiabetic group (4.18 vs 1.82% respectively); this difference was statistically significant (p = 0.001) along with higher AP/ET ratio (27.7 vs 19.3 respectively). The poorly controlled DM group had a higher prevalence of AP lesions compared with the well-controlled DM group (18.29 vs 9.21 respectively). This difference was statistically significant (p = 0.001); they also had a higher percentage of ET (5.55 vs 3.13% respectively) and AP/ ET ratio (32.0 vs 21.8% respectively).

This survey demonstrates a higher prevalence of AP in DM patients compared with nondiabetic group, with an increased prevalence of persistent chronic AP. Compared with a well-controlled diabetic group, a poor glycemic control may be associated with a higher prevalence of AP and increased rate of endodontic failures.

Counseling diabetic patients, particularly those with poor glycemic control, about the risk of failure of endodontic treatment can be part of planning management, which could include refereeing diabetic patients who need endodontic treatment for consultant care.

Smadi L. Apical Periodontitis and Endodontic Treatment in Patients with Type II Diabetes Mellitus: Comparative Cross-sectional Survey. J Contemp Dent Pract 2017;18(5):358-362.

Numerous appliances are present for the management of class II malocclusion. We have conducted a study to compare the clinical complications during treatment with either a removable mandibular acrylic splint (RMS) or with a cantilever Herbst (HC) appliance for the management of class II malocclusion.

This study consisted of records of 114 patients (61 males, 53 females), who were divided into two groups. Group I received RMS and group II received HC for the treatment of class II, Division 1 malocclusion. They were further subdivided according to the telescopic system used [Dentaurum type I or propulsor mandibular abzil (PMA)] and fixation mode (splint with crowns or GripTite bands). Patients’ clinical records were assessed to identify clinical complications.

The results of the study showed that the incidence of complications during treatment in both groups was statistically nonsignificant. The complications with either crown or band were also statistically nonsignificant. The Dentaurum group showed more susceptibility to complications than the PMA group.

The PMA telescopic system is more efficient as compared with Dentaurum. Complication resulting from Herbst appliance is independent type of appliance used and mode of fixation.

Herbst appliance is the treatment of choice for class II malocclusion.

Kanuru RK, Bhasin V, Khatri A, Dodda KK, Singh E, Grover S. Comparison of Complications in Removable Mandibular Acrylic Splint and Cantilever Herbst for Management of Class II Malocclusion: A Retrospective Study. J Contemp Dent Pract 2017;18(5):363-365.

An array of odontogenic lesions manifest in the maxillofacial region with variable presentations. The biological behavior of lesions, such as odontogenic keratocyst (OKC), dentigerous cyst (DC), and ameloblastoma (AM) always invite debate. Glucose transporter 1 (GLUT-1) is proven to be an indicator of metabolic behavior of several benign and malignant neoplasms.

The purpose of this study was to evaluate the expression of GLUT-1 in OKC, DC, and AM to understand their metabolic behavior.

Immunohistochemical expression of GLUT-1 was evaluated in each of the 15 cases of OKC, DC, and AM. The number of labeled cells, staining intensity, and membrane or cytoplasmic expressions were the parameters assessed and analyzed using chi-square test.

All cases showed positive GLUT-1 expression: 86.6% OKC showed more than 50% labeled cells followed by DC (40%) and AM (26.5%); 53.3% OKC showed strong intensity in comparison to AM, which showed weak intensity in 53.3% cases; 86.6% of OKCs showed both membrane and cytoplasmic expression followed by DC (40%) and AM (26.6%), whereas 73.3% of AM showed only membrane expression followed by DC (60%) and OKC (13.3%).

Odontogenic keratocyst was found out to be more metabolically active followed by DC and AM.

Bandyopadhyay A, Panda A, Behura SS, Ramachandra S, Dash KC, Mishra P. Glucose Transporter 1 Expression in Odontogenic Keratocyst, Dentigerous Cyst, and Ameloblastoma: An Immunohistochemical Study. J Contemp Dent Pract 2017;18(5):366-370.

Pregnancy is a natural process that may create some changes in different parts of the body including the oral cavity. These changes will lead to oral diseases if enough and timely care of oral cavity is not taken. Women may experience increased gingivitis or pregnancy gingivitis beginning in the second or third month of pregnancy that increases in severity throughout the duration of pregnancy. To motivate the patient toward oral health and implement the needed prophylactic measures, a longitudinal study was planned to observe the effect of oral health education during pregnancy on knowledge, attitude, practice, oral health status, and treatment needs (TNs) of pregnant women belonging to different socioeconomic groups.

A longitudinal study was conducted among 112 pregnant women belonging to different socioeconomic groups to assess the effect of oral health education on knowledge, attitude, practice, oral health status, and TNs. The demographic details, knowledge, attitude, and practice of pregnant women, and oral health status were collected through a predesigned questionnaire by a principal investigator through an interview. Oral health examination was carried out to assess oral health status using revised World Health Organization Proforma 1997, and oral health education was given through PowerPoint presentation to the participants in local language, i.e., Marathi, after collecting the baseline data. Reinforcement of oral health education and blanket referral was done at 14th week, and follow-up data were collected at 28th week of gestation. The demographic details, such as age, sex, education, occupation, income, and the questions based on knowledge, attitude, and practice among participants were analyzed using number, percentage, and mean.

At baseline, knowledge was limited, attitude was positive, while the practice was poor regarding oral health care during pregnancy in pregnant women belonging to different socioeconomic groups. After oral health education and blanket referral, at 28th week of gestation, knowledge regarding oral health care improved drastically, attitude toward oral health became more positive, whereas practice did not change much among all the pregnant women belonging to different socioeconomic groups, probably indicating sociocultural influences.

Intensive oral health education during pregnancy leads to drastic improvement in knowledge and attitude. Practice, gingival health, and the number of filled teeth also improved to some extent.

Regular oral health education programs should be conducted at community level among pregnant woman to reduce the burden of oral diseases.

Chawla RM, Shetiya SH, Agarwal DR, Mitra P, Bomble NA, Narayana DS. Knowledge, Attitude, and Practice of Pregnant Women regarding Oral Health Status and Treatment Needs following Oral Health Education in Pune District of Maharashtra: A Longitudinal Hospital-based Study. J Contemp Dent Pract 2017;18(5):371-377.

Osteoporosis is a systemic bone disease that can be prevented to some extent by awareness of its risk factors and related health behavior. The aim of this study was to collect information about knowledge and awareness of osteoporosis among women living in Jazan and to describe the type of food habits, sociodemographic characteristics, and other osteoporosis- related health behavior.

Self-administered questionnaires were used for data collection. The total participants were 546 women: 88 (16.1%) were health professionals and 458 (83.9%) non health professionals in the age range ≤20 to 50 years. Data collection was facilitated by a trained intern female doctor of dentistry whose task was to administer the distribution and collection of the questionnaires.

In total, 61.9% of health professionals and 54.3% of nonhealth professionals scored highly on knowledge of osteoporosis. The respondents of osteoporosis preventive food items were also highly knowledgeable with 50.6 and 52.6% of health and nonhealth professionals respectively. About 48.1% of health professional and 44.2% of nonhealth professional scored low on knowledge of osteoporosis behavior and attitude, vitamin supplements, and sunlight exposure. The age effect on osteoporosis knowledge was 33.8% in young age group and 30.7% in old age group.

Our study shows that Jazan women were highly knowledgeable and aware about osteoporosis and its related health behavior and that health professionals and nonhealth professionals were equally aware about osteoporosis-related health matters.

Awareness and preventive behavior of osteoporosis can prevent or slow the development of the disease.

Darout IA, Alamir A, Sultana S. Osteoporosis Knowledge and Related Health Behavior among Women in Jazan Region, Kingdom of Saudi Arabia. J Contemp Dent Pract 2017;18(5):378-382.

This ex vivo study evaluated the accuracy of the Root-ZX electronic apex locator (EAL) (J. Morita, Tokyo, Japan) and the NovApex (Forum Technologies, Rishon Lezion, Israel) in determining the working length (WL) during endodontic treatment.

Forty extracted single-rooted human teeth were selected for this study. The actual WL was measured with visual technique by a size #15 k-file under magnification. Then, the canal lengths were measured electronically with both Root-ZX and NovApex apex locators within ±0.5 and ±1 mm. Mean percentage of data was analyzed between groups using paired t-test, with a statistically significant level of p < 0.05.

The accuracy of NovApex apex locator was 85% within ±0.5 mm and 92.5% within ±1 mm. The accuracy of Root-ZX apex locator was 70% within ±0.5 mm and 97.5% within ±1 mm. There was no significant difference between the accuracy of the two EALs.

Both the NovApex and Root-ZX EALs are useful for measuring the WL with high accuracy.

Given the importance of accurate WL determination in the success of endodontic treatments, the accuracy of different apex locators should be evaluated.

Zand V, Rahimi S, Davoudi P, Afshang A. Accuracy of Working Length Determination using NovApex and Root-ZX Apex Locators: An in vitro Study. J Contemp Dent Pract 2017;18(5):383-385.

The aim of this study was to determine the treatment outcome of the use of a porcine monolayer collagen matrix (mCM) to augment peri-implant soft tissue in conjunction with immediate implant placement as an alternative to patient's own connective tissue.

A total of 27 implants were placed immediately in 27 patients (14 males and 13 females, with a mean age of 52.2 years) with simultaneous augmentation of the soft tissue by the use of a mCM. The patients were randomly divided into two groups: Group I: An envelope flap was created and mCM was left coronally uncovered, and group II: A coronally repositioned flap was created and the mCM was covered by the mucosa. Soft-tissue thickness (STTh) was measured at the time of surgery (T0) and 6 months postoperatively (T1) using a customized stent. Cone beam computed tomographies (CBCTs) were taken from 12 representative cases at T1. A stringent plaque control regimen was enforced in all the patients during the 6-month observation period.

Mean STTh change was similar in both groups (0.7 ± 0.2 and 0.7 ± 0.1 mm in groups I and II respectively). The comparison of STTh between T0 and T1 showed a statistically significant increase of soft tissue in both groups I and II as well as in the total examined population (p<0.001). The STTh change as well as matrix thickness loss were comparable in both groups (p>0.05). The evaluation of the CBCTs did not show any signs of resorption of the buccal bone plate.

Within the limitations of this study, it could be concluded that the collagen matrix used in conjunction with immediate implant placement leads to an increased thickness of peri-implant soft tissue independent of the flap creation technique and could be an alternative to connective tissue graft.

The collagen matrix used seems to be a good alternative to patient's own connective tissue and could be used for the soft tissue augmentation around dental implants.

Zafiropoulos GG, John G. Use of Collagen Matrix for Augmentation of the Peri-implant Soft Tissue at the Time of Immediate Implant Placement. J Contemp Dent Pract 2017;18(5):386-391.

The aim of this study is to evaluate the in vitro antimicrobial activity of plant extracts and chemical fractions of Sideroxylon obtusifolium T.D. Penn on Streptococcus mutans, Streptococcus oralis, Streptococcus salivarius, Streptococcus parasanguinis, and Candida albicans as well as to identify the chemical classes found in the bioactive extracts possessing better activity.

Freeze-dried hydroalcoholic extracts of the bark and leaves (LC and LF respectively) and ethanol extracts of bark and leaves (EC and EF respectively) of S. obtusifolium were assessed for antimicrobial potential by determining the minimum inhibitory concentration (MIC), minimum bactericidal concentration, and minimum fungicidal concentration (MFC). The tests were performed by microdilution method (in triplicate) in three independent experiments. Phytochemical characterization was performed by quantification of total polyphenols, total flavonoids, and condensed tannins.

The EC extract presented weak antimicrobial potential on the growth of S. mutans (MIC = 1000 µg/mL); all extracts showed moderate inhibitory activity on the growth of C. albicans (MIC = 500 µg/mL). The dichloromethane and n-butanol fractions of LF extracts showed moderate growth inhibitory activity (MIC = 250 µg/mL) on C. albicans and fungicide potential (MFC/MIC = 2). The phytochemical characterization revealed a predominance of total polyphenols (EC = 29.23%; LC = 25.98%) and condensed tannins (LC = 38.84%; LF = 17.78%).

The dichloromethane and n-butanol fractions of S. obtusifolium LF extract showed antifungal activity against C. albicans, with the potential for bioprospection of phytocompounds for the treatment of periodontal fungal diseases caused by this microorganism. The effect may be related to phytochemical compounds from the polyphenol and condensed tannin classes.

Research for new oral microbial disease treatment alternatives in bioactive compounds from medicinal plants is of clinical relevance and scientific interest since many therapeutic antifungal agents do not obtain the effectiveness expected due to microbial resistance, or to adverse effects on human tissues.

Sampaio TPD, Cartaxo-Furtado NAO, de Medeiros ACD, Alves HS, Rosalen PL, Pereira JV. Antimicrobial Potential of Plant Extracts and Chemical Fractions of Sideroxylon obtusifolium (Roem. & Schult.) T.D. Penn on Oral Microorganisms. J Contemp Dent Pract 2017;18(5):392-398.

The objectives of this study were to compare the qualitative and quantitative profiles of herpes simplex virus type I (HSV-1) in implant surfaces between participants with periimplantitis (PI) and Healthy peri-implant tissues and to quantitatively assess the relation between HSV-1 and periopathogens inside the microbiological profile associated with PI.

A total of 40 patients with PI and 40 with healthy peri-implant tissues (HI) were recruited. Plaque samples from peri-implant sulcus and internal implant connections were analyzed using quantitative real-time polymerase chain reaction to detect and quantify HSV-1 and periodontopathogens. Frequencies of detection and levels of microorganisms were compared between PI and HI; the frequencies and levels of periodontopathogens were compared between HSV-1+ and HSV-1- PI to assess qualitative relations between HSV-1 and bacteria. Correlation between HSV-1 and periodontopathogens levels was assessed in PI and HI.

A total of 77 dental implants affected by PI, and 113 HIs were included. The HSV-1 prevalence was slightly higher in PI compared with controls (33.3 vs 23.8%; p > 0.05); HSV-1 was detected in external samples more frequently compared with internal samples. The HSV-1-positive patients revealed higher median loads of Prevotella intermedia (Pi) and Campylobacter rectus (Cr) compared with HSV-1-negative patients. In the PI group, a significant positive correlation was evidenced between HSV-1 and Tannerella forsythia, Parvimonas micra (Pm), Fusobacterium nucleatum, and Cr levels, while in the HI, positive correlation between HSV-1 and Aggregatibacter actinomycetemcomitans, Pi, and Pm was established.

The HSV-1 prevalence cannot be used to identify PI. The HSV-1 was found in similar levels of PI and HI patients after an average of 6 years of loaded implants. The HSV-1 prevalence cannot be used to identify implants with or without the presence of PI.

Although HSV-1 is detected in PI site, HSV-1 may represent an unspecific indicator for the host response to the bacterial challenge observed in PI.

Parthiban S, Ahmed N, Ramakrishnan T, Balakumar V, Raja M, Shekhar H. Herpes Simplex 1 and Periopathogen Role in Peri-implantitis. J Contemp Dent Pract 2017;18(5):399-404.

Replacement of missing teeth by dental implants is one of the most common methods employed these days. Because of significant advancement in the design of implants and modifications in the procedure of dental implant surgery, the survival rate of the dental implants has reached up to approximately 95%. Osseointegration is one of the important factors affecting the survival of dental implants. Apart from these, the body's physiologic alterations can also predispose the dental implants for failure. Diabetes is one such metabolic disease characterized by abnormal or delayed wound healing. Hence, we assessed the clinicomicrobial and salivary profile of diabetic patients undergoing rehabilitation by dental implants.

This study included diabetic patients who underwent dental implant surgeries for prosthetic rehabilitation. Follow-up records of the patients’ up to 1 year were maintained. Various clinicoradiographic and periodontal parameters were measured at various time intervals during follow-up time; 25 mL of salivary and blood sample was taken from all the subjects and was sent to the laboratories for assessment of various salivary biomarkers. All the results were analyzed by Statistical Package for the Social Sciences software.

The mean level of interleukin-â at baseline time was found to be 2.38 and 2.21 in diabetic group and control group respectively. While comparing the levels of osteoprotegerin in both study groups, a significant correlation was obtained. In diabetic and control group, 62 and 61 years was the mean age of the patients respectively. No significant correlation was obtained while comparing the microbial flora of diabetic and control group.

In both diabetic and nondiabetic patients, similar microbial, salivary marker, and clinicoradiological patterns were seen.

Diabetic patients who maintain their body's metabolic rate show similar success rate of dental implants as seen in nondiabetic patients.

Raghav D, Alqahtani F, Albaker FJ, Bhagat TV, Kola Z. Intricate Assessment and Evaluation of Long-term Implant Success as affected by Clinicomicrobial and Salivary Diagnostics in Type II Diabetic Patients: A Longitudinal Study. J Contemp Dent Pract 2017;18(5):405-409.

Chin cup (CC) therapy has been used as the traditional appliance for treating class III malocclusion during mixed dentition period. The aim of this study was to investigate the effect of CC on the improvement of skeletal and dentoalveolar skeletal changes in class III patients during mixed dentition stage.

A total of 30 patients (7–9 years old) with skeletal class III malocclusion were selected based on clinical and cephalometric examination. Out of 30 patients, 20 underwent CC therapy. All orthodontic records and measurements were taken before and after treatment. Similar records were collected from the control group. The lateral cephalometric films were traced before and after treatment and analyzed.

There was a significant improvement in maxillary and the mandibular skeletal measurements after CC therapy. Improvement of ANB angle and an increase in Wits appraisal have been detected in the treated group according to intermaxillary skeletal variables.

The study concluded that the CC therapy is effective for correcting skeletal class III malocclusion along with positive changes in the dentoskeletal variables during the mixed dentition stage.

Al-Khalifa HN, Hashem MI, Alanazi KJ, Anil S. Orthopedic Effect of Chin Cup during Mixed Dentition Stage. J Contemp Dent Pract 2017;18(5):410-414.

Mohammadi Z, Jafarzadeh H, Shalavi S, Kinoshita JI. Unusual Root Canal Irrigation Solutions. J Contemp Dent Pract 2017;18(5):415-420.