About Video

This video highlights regenerative medicine preparation and execution. After the patient has been prepared for surgery but prior to any physical trauma, blood must be drawn that will be used to prepare the platelet-rich plasma (PRP). It is critical to have the PRP drawn prior to any body insult because otherwise the growth factors contained within the blood will rush to the site of injury rather than be drawn out of the blood to be used for reinjection elsewhere. In the case of PRP, it is postulated that the ideal effect of the PRP is achieved with a concentration of between 1.8 and 2.5 times physiologic levels of normal circulating serum platelets. The Angel system comes equipped with 60-mL syringes that draw up blood with a butterfly needle. Using 60 mL of whole blood, dilute down the very concentrated PRP output to a solution of about 18 mL, which yields a platelet concentration of 2.0 × physiologic. Typically draw two 60-mL syringes in most surgical cases to be able to obtain approximately 35 mL of PRP at a concentration of about 2.0 × physiologic. The method by which the Angel system works is that there is a super-rich component of PRP, typically about 3–4 mL, with a large residual of platelet-poor plasma (PPP). When the 3–4 mL of super-rich plasma is diluted down to a total of 35 mL, the yield reaches the aforementioned 2 × physiologic platelet concentration. The remainder of the PPP can also be used, as will be described in the following section. Activated PPP (activated either by mechanical trauma like donor harvesting or recipient-site creation or chemically like by calcium gluconate) has a very excellent bio­activity that is only shy of PRP. At this time, use PPP for the donor scar and at times, inject it into any of the fringes around the crown or lateral humps where, would like to stimulate hair growth. When the PRP has been titrated to the above-referenced concentration and drawn up into syringes, the total 35 mL of PRP is injected into a sterile cup and mixed with 100 mg of fine-particle powder ACell Matristem Micromatrix (AMM) (the Micromatrix refers to the powder version of Matristem, which is what use exclusively) and stirred using a sterile tongue depressor. The PRP mixed with AMM is then drawn up into seven 5-mL syringes. These syringes are kept aside for later use, as will be outlined in the appropriate sections below.